A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis

Renata Los; Rafal Sawicki; Marek Juda; Magdalena Stankevič; Pawel Rybojad; Marek Sawicki; Anna Malm; Grazyna Ginalska

doi:10.1111/j.1574-6968.2010.02050.x

A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis

Abstract

The collection of 146 Staphylococcus epidermidis strains isolated from the nasopharynx of lung cancer patients has been studied for the ability of slime secretion and biofilm formation using the Congo red agar (CRA) test and the microtiter plate (MtP) method, respectively. The prevalence of the icaAD and the aap genes was also analyzed. Some isolates (35.6%) were biofilm positive by the MtP method, while 58.9% of isolates exhibited a slime-positive phenotype by the CRA test. The sensitivities of the CRA test evaluated using the MtP method as a gold standard of biofilm production were 73.1%, 97.3% and 13.3% for all the strains screened, ica-positive and ica-negative strains, respectively. The genotype ica(+)aap(+) was correlated with a strong biofilm-producer phenotype. Interestingly, some of the ica(-)aap(-) isolates could also form a biofilm. The correlation between the presence of icaAD genes and the biofilm-positive phenotype by the MtP method as well as slime production by the CRA test was statistically significant (P<0.0001). However, some S. epidermidis strains possess the potential ability of ica-independent biofilm formation; thus, further studies are needed to determine reliable, short-time criteria for an in vitro assessment of biofilm production by staphylococci.

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Authors (8)

Renata Los
- Department of Pharmaceutical Microbiology, Medical University of Lublin
Rafal Sawicki
- Department of Biochemistry and Biotechnology, Medical University of Lublin
Marek Juda
- Department of Pharmaceutical Microbiology, Medical University of Lublin
Magdalena Stankevic (formerly: Magdalena Stankevic) dr inż.
- Department of Biochemistry and Biotechnology, Medical University of Lublin
Pawel Rybojad
- Department of Thoracic Surgery, Medical University of Lublin
Marek Sawicki
- Department of Thoracic Surgery, Medical University of Lublin
Anna Malm
- Department of Pharmaceutical Microbiology, Medical University of Lublin
Grazyna Ginalska
- Department of Biochemistry and Biotechnology, Medical University of Lublin

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Category:: Articles
Type:: artykuły w czasopismach
Published in:: FEMS MICROBIOLOGY LETTERS no. 310, pages 97 - 103,
ISSN: 0378-1097
Language:: English
Publication year:: 2010
Bibliographic description:: Los R., Sawicki R., Juda M., Stankevic M., Rybojad P., Sawicki M., Malm A., Ginalska G.: A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis// FEMS MICROBIOLOGY LETTERS -Vol. 310,iss. 2 (2010), s.97-103
DOI:: Digital Object Identifier (open in new tab) 10.1111/j.1574-6968.2010.02050.x
Verified by:: Gdańsk University of Technology