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Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status
Abstrakt
OBJECTIVES: This study aims to examine biofilm formed on vascular prostheses by Staphylococcus epidermidis with different ica and aap genetic status, and to evaluate the effect of antibiotic-modified prostheses on bacterial colonization. METHODS: Biofilm formation was determined using fluorescence microscopy imaging. Quantitative analysis was conducted using the biofilm coverage ratio (BCR) calculations. RESULTS: Our investigations prove that the BCR method with fluorescent dye enabled an accurate assessment of biofilm coverage and comparison of the obtained results. The ica+ aap+ strains formed a biofilm on all of the examined vascular prostheses. Uni-Graft(®) modified with covalently immobilized amikacin was effective in preventing bacterial adherence. CONCLUSIONS: Molecular biology techniques combined with phenotype studies give a broad insight into biofilm formation mechanisms. On the other hand, fluorescence microscopy imaging along with BCR calculations are reliable and simple tools to quantitatively estimate biofilm formation, as well as the effectiveness of antimicrobial prosthesis modification.
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5
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Autorzy (6)
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Joanna Golus
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Rafal Sawicki
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Renata Los
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Anna Malm
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Grazyna Ginalska
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
nr 36,
strony 105 - 112,
ISSN: 0391-3988 - Język:
- angielski
- Rok wydania:
- 2013
- Opis bibliograficzny:
- Golus J., Stankevic M., Sawicki R., Los R., Malm A., Ginalska G.: Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status// INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS -Vol. 36,iss. 2 (2013), s.105-112
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.5301/ijao.5000157
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 90 razy
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