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MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products
Abstract
The abundance of protein markers in different types of meat cuts was explored in the context of authentication of raw meat (pork, beef and chicken) and processed meat products. Peptides originating from myoglobin (Mb) and myosin (My) were analyzed using multiple reaction monitoring mass spectrometry (MRM–MS). Analytical protocol was optimized for good repeatability (CV < 10%) and high sensitivity. The MS signal intensity of Mb marker peptides in raw pork depended significantly on the cut type (e.g. ham vs knuckle). Importantly, a similar pattern in the abundance of the marker peptides was found for processed meat products made of different types of pork cuts, despite the food processing applied. This suggests the protocol can be used for authentication of raw pork cuts and processed products made of different cuts of pork. More uniform contents of Mb markers were found in raw beef cuts, and for My markers in raw chicken cuts.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
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FOOD CHEMISTRY
no. 283,
pages 367 - 374,
ISSN: 0308-8146 - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Nalazek-Rudnicka K., Kłosowska-Chomiczewska I., Wasik A., Macierzanka A.: MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products// FOOD CHEMISTRY. -Vol. 283, (2019), s.367-374
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.foodchem.2019.01.007
- Verified by:
- Gdańsk University of Technology
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