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Description
Enzyme of fungal L-methionine biosynthetic pathway: O-acetylhomoserine sulfhydrylase (Met15p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding a native protein CaMET15 or recombinants with oligo-His tags added either at the C- (CaMET15CH) or N-end (CaMET15NH ) were used for overexpression of the cloned genes in a IPTG inducible Tabor-Studier system.
This dataset contains the description of methodology, used E. coli cells, overproduction conditions and three image files with the results of SDS-PAGE analysis of CaMet15p, CaMet15NHp and CaMet15CHp overproduction in E. coli cells.
The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
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CC BYAttribution - Raw data:
- Data contained in dataset was not processed.
Details
- Year of publication:
- 2023
- Verification date:
- 2023-03-27
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/y9xa-5w80 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
- dataset Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
- dataset Antifungal activity of L-homoserine O-acetyltransferase (CaMet2p) inhibitors.
- dataset L-Homoserine O-acetyltransferase (CaMet2p) activity and inhibition
- dataset Inserts amplification for knockout cassette construction
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