Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions. - Open Research Data - MOST Wiedzy

Search

Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.

Description

Enzyme of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding a native protein CaMet2p or recombinants with oligo-His tags added either at the C- (CaMet2CHp) or N-end (CaMet2NHp) were used for overexpression of the cloned genes in a IPTG inducible Tabor-Studier system.

This dataset contains the description of methodology, used E. coli cells, overproduction conditions and two image files with the results of SDS-PAGE analysis of CaMet2p, CaMet2NHp and CaMet2CHp overproduction in E. coli cells.

The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).

Dataset file

Overproduction of Met2p native and His-tag versions.zip
0.0 B, S3 ETag , downloads: 1
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5
download file Overproduction of Met2p native and His-tag versions.zip

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
Raw data:
Data contained in dataset was not processed.

Details

Year of publication:
2022
Verification date:
2022-06-21
Dataset language:
English
Fields of science:
  • Chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/kgrw-6e43 open in new tab
Funding:
Verified by:
Gdańsk University of Technology

Keywords

References

Cite as

seen 12 times