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A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry
Abstract
Ibuprofen is widely used in human and veterinary medicine for the treatment of chronic pain as well as rheumatic and musculoskeletal disorders. However, t he anal- gesic and anti-inflammatory properties of Ibuprofen have contributed to frequent drug abuse in equestr ian s ports. A sensitive and rapid gas chromatography with tandem mass spectrometry based method with a simple liquid-liquid extraction and deriva- tization requiring 200 μL volume of sample and 2 mL of extraction solvent for the simultaneous determination of ibuprofen and its metabolites was developed. The pro- posed procedure was optimized and validated according to the principles for bioana- lytical methods. The assay achieved satisfactory validation parameters, namely, recov- ery (92.2–105%), interday accuracy (92.5–106%), and precision (0.3–4.4%) for all investigated compounds as well as limits of quantification of 50 ng/mL for ibuprofen, 2-hydroxyibuprofen, and carboxyibuprofen, 25 ng/mL for 1-hydroxyibuprofen and 100 ng/mL for 3-hydroxyibuprofen. The applicability of the method was evaluated by the analysis of five real urine samples collected from different horses after drug administration. In view of the low limits of quantification, high selectivity, repeata- bility, and recovery, t he procedure can be utilized for laboratory applications, includ- ing the control of ibuprofen abuse in equestrian sports for anti-doping purposes and drug/pharmaceutical mentality investigations.
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Authors (6)
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Ewa Kłodzińska dr. hab.
- Instytut Sportu Zakład Chemii Analityznej i Analiz Istrumentalnych
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Robert Wrzesień
- Warszawski Uniwersytet Medyczny Centralne Laboratorium Zwierząt Doświadczalnych
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Barbara Bobrowska-Korczak
- Warszawski Uniwersytet Medyczny Katedra Bromatologii
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF SEPARATION SCIENCE
no. 41,
pages 3881 - 3891,
ISSN: 1615-9306 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Waraksa E., Woźniak M. K., Kłodzińska E., Wrzesień R., Bobrowska-Korczak B., Namieśnik J.: A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry// JOURNAL OF SEPARATION SCIENCE. -Vol. 41, iss. 20 (2018), s.3881-3891
- DOI:
- Digital Object Identifier (open in new tab) 10.1002/jssc.201800614
- Verified by:
- Gdańsk University of Technology
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