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A selection of the optimal eluent components for separation of carnivorous plants metabolites by planar chromatography chromatography
Abstract
Abstract: The development of the new procedures of the separation components with the complicated chemical structure from natural complex matrix by column chromatography is very difficult and in many cases expensive. Therefore to pre-select the conditions of the separation process the use of thin layer chromatography (TLC) is proffered. The possibility of proceeding the chromatographic process for several mixtures on single plate, significantly reduces time and amount of the reagents needed for completing the "set" of the separations in the tested chromatographic conditions. Another advantage is the simplicity of using a so-called orthogonal separation - a separation of few mixtures in the same TLC plate in two perpendicular directions with use of two different eluents. This allows doubling the retention data of separated components achieved for analyzed stationary phase. The use of suitably chromatographic system in connection with the specific biological and chemical methods of detection, allows getting preliminary information about presence and retentions parameters of biologically active components occurring in separated mixtures.The paper presents an analysis of the retention data obtained for reversed phase TLC with two-, three-component component eluent for selection of the optimal selectivity of the chromatographic system. In the paper a results of comparison of the sorption properties of different stationary phases are also provided. An influence of the acid addition to the eluent on the so-called peak "tailing" of the flavonoids is also discussed
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- Category:
- Conference activity
- Type:
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Title of issue:
- 14th International Symposium of Students and Young Mechanical Engineers :''Advances in Chemical and Mechanical Engineering". - Vol. 1/2, Gdansk, 5th-7th May 2011 strony 275 - 282
- Language:
- English
- Publication year:
- 2011
- Bibliographic description:
- Kamiński M., Jaszczołt M., Boczkaj G., Lewandowski A.: A selection of the optimal eluent components for separation of carnivorous plants metabolites by planar chromatography chromatography// 14th International Symposium of Students and Young Mechanical Engineers :''Advances in Chemical and Mechanical Engineering". - Vol. 1/2, Gdansk, 5th-7th May 2011/ ed. ed. C. Fijało. - Faculty Mechanical Engineering, Chemical Faculty Gdansk University of Technology. Gdańsk: , 2011, s.275-282
- Verified by:
- Gdańsk University of Technology
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