Liquid chromatography techniques for separation of flavonoids from Droseraceae

Mariusz Jaszczołt; Anita Skrzypczak; Aleksandra Królicka; Lewandowski Aleksander; Marian Antoni Kamiński

doi:10.1055/s-0031-1282248

Liquid chromatography techniques for separation of flavonoids from Droseraceae

Abstract

Flavonoids which are presented in insectivorous plants of the Droseraceae family have wide range of advantageous properties i.e. an antioxidant, anti-inflammatory and antimicrobial activities, antitumor activity was reported as well.The purpose of the research was to develop the most favourable conditions for liquid chromatographic separation and identification of myricetin and quercetin in extracts of insectivorous plants. In the research a methanol and chloroform extracts of the Drosera binata Labill., Drosera capensis L., Drosera aliciae Raym.-Hamet and Dionea muscipula Ellis cultivated in vitro were used. In the first stage of the research an optimal composition of the eluent for selective separation of the components included in the extracts was selected. The thin layer chromatography (TLC) in the reversed phase and hydrophilic interaction chromatography (HILIC) conditions was used for selection of optimal chromatographic systems. In the second stage of the research a high performance liquid chromatography (HPLC) in RP and HILIC optimal conditions was used for detailed characteristic of analysed mixtures.The results show that thin layer chromatography is helpful technique for pre selection components of the eluent to separation of the flavonoids from complex herbal matrix. In the paper there is reported that application gradient elution is more preferable than isocratic elution in HPLC techniques.Acknowledgement: State Commitee for Scientific Research, Grant No N N405 3757 37. This research work was supported by the European Union in the framework of the European Social Fund. The system project of the Pomorskie Voivodeship "InnoDoktorant - Scholarships for PhD students, II edition".

Citations

0

CrossRef
0

Web of Science
0

Scopus

Authors (5)

Mariusz Jaszczołt mgr inż.
Anita Skrzypczak
Aleksandra Królicka
Lewandowski Aleksander
Marian Antoni Kamiński prof. dr hab. inż.

Cite as

Full text

full text is not available in portal

full content of the article see on external site open in new tab

Keywords

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: PLANTA MEDICA no. 77, pages 1275 - 1275,
ISSN: 0032-0943
Language:: English
Publication year:: 2011
Bibliographic description:: Jaszczołt M., Skrzypczak A., Królicka A., Aleksander L., Kamiński M.: Liquid chromatography techniques for separation of flavonoids from Droseraceae// PLANTA MEDICA. -Vol. 77, nr. iss. 12 (2011), s.1275-1275
DOI:: Digital Object Identifier (open in new tab) 10.1055/s-0031-1282248
Verified by:: Gdańsk University of Technology