Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants - Publication - Bridge of Knowledge

Your account

login

Search

Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants

Abstract

This study demonstrates the potential of a commercially available derivatization instrument coupled with HPLC for separation and on-line determination of antioxidants detected with widely used screening reagents - DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) or FCR (Folin-Ciocalteu's phenol reagent ). As the result of optimization, key parameters of the analytical procedure, were established: concentrations of derivatization reagents 1.5 mmol/L and 2.1 mmol/L in methanol for DPPH and ABTS, respectively and 40 % (v/v) FCR solution in water; reactor temperatures 130 oC for ABTS and FCR and 50 oC in the case of DPPH. In order to validate these procedures, Trolox equivalent antioxidant capacities (TEAC) for standard antioxidants obtained by post-column derivatization (on-line) and typical batch colorimetric methods (off-line) were compared; the corresponding measurements were found to be strongly correlated (Pearson coefficient: 0.973, 0.922, 0.853 for DPPH, ABTS and FCR, respectively). The results obtained for standard antioxidants and real fruit sample - aronia extract, confirm the applicability of the proposed system to the on-line detection of antioxidants. Its great advantage compared to current routine methods is that it provides chromatographic profiles and corresponding fingerprints of antioxidants (including unknown ones) along with quantitative determination of antioxidative potential, both - total and exhibited by individual compounds.

Citations

  • 2 9

    CrossRef

  • 0

    Web of Science

  • 2 9

    Scopus

Authors (4)

Cite as

Full text

download paper
downloaded 173 times
Publication version
Accepted or Published Version
License
Creative Commons: CC-BY-NC-ND open in new tab

Keywords

Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
JOURNAL OF FOOD COMPOSITION AND ANALYSIS no. 24, edition 7, pages 1073 - 1080,
ISSN: 0889-1575
Language:
English
Publication year:
2011
Bibliographic description:
Kusznierewicz B., Piasek A., Bartoszek-Pączkowska A., Namieśnik J.: Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants// JOURNAL OF FOOD COMPOSITION AND ANALYSIS. -Vol. 24, iss. 7 (2011), s.1073-1080
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.jfca.2011.01.010
Bibliography: test
  1. Bandoniene, D., Murkovic, M., 2002. On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domes- tica L.). Journal of Agricultural Food Chemistry 50, 2482-2487. open in new tab
  2. Bartasiute, A., Westerink, B.H.C., Verpoorte, E., Niederlander, H.A.G., 2007. Improv- ing the in vivo predictability of an on-line HPLC stable free radical decoloration assay for antioxidant activity in methanol-buffer medium. Free Radical Biology and Medicine 42, 413-423. open in new tab
  3. Borges, G., Degeneve, A., Mullen, W., Crozier, A., 2010. Identification of flavonoid and phenolic antioxidants in black currants, blueberries, raspberries, red currants, and cranberries. Journal of Agricultural Food Chemistry 58, 3901-3909. open in new tab
  4. Cardenosa, R., Mohamed, R., Pineda, M., Aguilar, M., 2002. On-line HPLC detection of tocopherols and other antioxidants through the formation of a phosphomolybdenum complex. Journal of Agricultural Food Chemistry 50, 3390-3395. open in new tab
  5. Dapkevicius, A., van Beek, T.A., Niederlander, H.A.G., 2001. Evaluation and compar- ison of two improved techniques for the on-line detection of antioxidants in liquid chromatography eluates. Journal of Chromatography A 912, 73-82. open in new tab
  6. Exarchou, V., Fiamegos, Y.C., van Beek, T.A., Nanos, C., Vervoort, J., 2006. Hyphenated chromatographic techniques for the rapid screening and identification of anti- oxidants in methanolic extracts of pharmaceutically used plants. Journal of Chromatography A 1112, 293-302. open in new tab
  7. Huang, D., Ou, B., Prior, R.I., 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural Food Chemistry 53, 1841-1856. open in new tab
  8. Koleva, I.I., Niederlander, H.A.G., van Beek, T.A., 2000. An on-line HPLC method for detection of radical scavenging compounds in complex mixtures. Analytical Chemistry 72, 2323-2328. open in new tab
  9. Koleva, I.I., Niederlander, H.A.G., van Beek, T.A., 2001. Application of ABTS radical cation for selective on-line detection of radical scavengers in HPLC eluates. Analytical Chemistry 73, 3373-3381. open in new tab
  10. Kool, J., Van Liempd, S.M., Harmsen, S., Schenk, T., Irth, H., Commandeur, J.N.M., Vermeulen, N.P.E., 2007. An on-line post-column detection system for the detection of reactive-oxygen species-producing compounds and antioxidants in mixtures. Analytical and Bioanalytical Chemistry 388, 871-879. open in new tab
  11. Mnatsakanyan, M., Goodie, T.A., Conlan, X.A., Francis, P.S., McDermott, G.P., Barnett, N.W., Shock, D., Gritti, F., Guiochon, G., Shalliker, R.A., 2010. High performance liquid chromatography with two simultaneous on-line antioxidant assays: evaluation and comparison of espresso coffees. Talanta 81, 837-842. open in new tab
  12. Milasiene, R., Sawicka, K., Kornysova, O., Ligor, M., Maruska, A., Buszewski, B., 2007. Evaluation of antioxidactivity of green and black tea (Camellia sinesis) and rooibos (Aspalathus linearis) tea extracts by means of HPLC with reaction detector. Ars Separatoria Acta 5, 27-33.
  13. Moon, J.K., Shibamoto, T., 2009. Antioxidant assays for plant and food components. Journal of Agricultural Food Chemistry 57, 1655-1666. open in new tab
  14. Niederlander, H.A.G., van Beek, T.A., Bartasiute, A., Koleva, I.I., 2008. Antioxidant activity assays on-line with liquid chromatography. Journal of Chromatography A 1210, 121-134. open in new tab
  15. Perez-Bonilla, M., Salido, S., van Beek, T.A., Linares-Palomino, P.J., Altarejos, J., Nogueras, M., Sanchez, A., 2006. Isolation and identification of radical scaven- gers in olive tree (Olea europaea) wood. Journal of Chromatography A 1112, 311-318. open in new tab
  16. Shi, S.-Y., Zhou, H.-H., Zhang, Y.-P., Jiang, X.-Y., Chen, X.-Q., Huang, K.-L., 2009. Coupling HPLC to on-line, post-column (bio)chemical assays for high-resolution screening of bioactive compounds from complex mixtures. Trends in Analytical Chemistry 28, 865-877. open in new tab
  17. Stalmach, A., Mullen, W., Nagai, C., Crozier, A., 2006. On-line HPLC analysis of the antioxidant activity of phenolic compounds in brewed, paper-filtered coffee. Brazilian Journal of Plant Physiology 18, 253-262. open in new tab
  18. Wu, J.-H., Huang, C.-Y., Tung, Y.-T., Chang, S.-T., 2008. Online RP-HPLC-DPPH screening method for detection of radical-scavenging phytochemicals from flowers of Acacia confusa. Journal of Agricultural and Food Chemistry 56, 328-333. open in new tab
Verified by:
Gdańsk University of Technology

seen 133 times

Recommended for you

The optimisation of analytical parameters for routine profiling of antioxidants in complex mixture by HPLC coupled post-column derivatisation

2011

Post-Chromatographic Derivatization Coupled with Mass Spectrometry as a Method of Profiling and Identification of Antioxidants; Ligustrum vulgare Phytocomplex as an Example

2023

Comparison of the content of phenolic compounds and antioxidant activity of white, red and black currants (Ribes sp.) extracts

2014

Phenolic composition and antioxidant properties of polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization

2012
Meta Tags