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The optimisation of analytical parameters for routine profiling of antioxidants in complex mixture by HPLC coupled post-column derivatisation
Abstract
Introduction: The wide application of natural and artificial antioxidants in food, cosmetic and pharmaceutical industry as well as the recognition of the importance of food antioxidants for supporting human health created demand for reliable and industrially applicable methods of determining antioxidative activity. This requirement can be fullfilled with the recently proposed HPLC-post-column derivatisation approach enabling the rapid measurements of antioxidative potential along with profiling antioxidants in complex mixtures.Objective: To employ the commercially available post-column derivatisation device for the on-line detection of antioxidants and to opimise analytical parameters enabling its application for routine profiling of antioxidants in complex mixtures as those of plant or food origin.Methodology: The mixtures of standards and fruit extracts were resolved using optimised HPLC method followed by the on-line derivatisation of analytes in PCX post-column derivatisation instrument. Such parameters as type of indicator reagent (ABTS, DPPH, Folin-Ciocalteu), its concentration and temperature of derivatisation reaction were investigated. The determinations of TE values were compared with those obtained by corresponding bulk spectrophotometric tests. Results: The study confirms that the commericially HPLC-coupled post-column derivatisation instruments are suitable for routine on-line detection of antioxidants in complex mixtures and the determination of their TE values. The analytical parameters optimised here appear to represent a ready-to-use toolbox for the food and pharmaceutical industries, enabling the monitoring of bioactive substances along the production line and during storage, and the characterisation of plant material by creating chromatographic profiles supplemented with antioxidant fingerprints.
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- Copyright (2011 John Wiley & Sons, Ltd.)
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PHYTOCHEMICAL ANALYSIS
no. 22,
edition 5,
pages 392 - 402,
ISSN: 0958-0344 - Language:
- English
- Publication year:
- 2011
- Bibliographic description:
- Kusznierewicz B., Piasek A., Bartoszek-Pączkowska A., Namieśnik J.: The optimisation of analytical parameters for routine profiling of antioxidants in complex mixture by HPLC coupled post-column derivatisation// PHYTOCHEMICAL ANALYSIS. -Vol. 22, iss. 5 (2011), s.392-402
- DOI:
- Digital Object Identifier (open in new tab) 10.1002/pca.1294
- Verified by:
- Gdańsk University of Technology
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