Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )

Joanna Cieślewicz; Marcin M. Kamiński; Marian Antoni Kamiński

doi:10.1002/jssc.201800506

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )

Abstract

The review presents general principles for choosing optimal conditions for ecdysteroid separation, identification, and isolation using HPLC/TLC techniques in RP, NP- HILIC or NP modes. Analytics of ecdyteroids pose a still insufficiently resolved problem. Plant-derived ecdysteroids are a point of interest of pharmaceutical industry and sport medicine due to their postulated adaptogenic and anabolic properties. In insects, ecdysteroids regulate larval transformation. Maral root (Rhaponticum carthamoides, Leuzea carthamoides), traditional Siberian folk-medicine plant used as stimulant to boost overall health and fitness, is a particularly rich source of a wide variety of phytoecdysteroids. The similarity of molecular structures of ecdysteroids present in its extracts together with high content of unrelated compounds of similar chromatographic characteristics makes optimization of separation, identification and isolation of ecdysteroids a difficult analytical task. In that respect, two-dimensional separations, two-dimensional separations, 2D HPLC or 2D TLC, could be of use. For identification, the hyphenated techniques are particularly important. Thus, comprehensive overview of MS spectral parameters of ecdysteroids is provided. Described principles could easily be applied for separation of ecdysteroids in extracts from other sources. They are also useful for development of separation procedures for isolation of ecysteroids in preparative-scale applications.

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Authors (3)

Joanna Cieślewicz (formerly: Joanna Głazowska)
Marcin M. Kamiński
Marian Antoni Kamiński prof. dr hab. inż.

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Keywords

LEUZEA CARTHAMOIDES , LIQUID CHROMATOGRAPHY OF PLANT SECONDARY METABOLITES, MARAL ROOT, PHYTOECDYSTEROIDS, RHAPONTICUM CARTHAMOIDES

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF SEPARATION SCIENCE no. 41, pages 4304 - 4314,
ISSN: 1615-9306
Language:: English
Publication year:: 2018
Bibliographic description:: Głazowska J., Kamiński M. M., Kamiński M. A.: Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )// JOURNAL OF SEPARATION SCIENCE. -Vol. 41, iss. 23 (2018), s.4304-4314
DOI:: Digital Object Identifier (open in new tab) 10.1002/jssc.201800506
Verified by:: Gdańsk University of Technology