Search
Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains - Verification of beetroot samples
Abstract
This study presents methods that can be used to assess the health quality of products containing betalains. The paper compares and verifies data on the phytochemical composition of three different pigmented beetroot cultivars using spectrophotometric, HPLC-DAD, HPTLC and LC-Q-Orbitrap-HRMS techniques. Additionally, we compared the total antioxidant activity in both the cell-free and cellular systems. Betalain contribution to antioxidant activity was also determined using post-column derivatization and it was found that in the case of red beetroot it is about 50%. Photometric measurements are recommended for a simple and inexpensive analysis of the total betacyanin and betaxanthin content. Liquid chromatography techniques produced more precise information on phytochemical composition in the tested samples. The combination of liquid chromatography with high-resolution mass spectrometry produced the largest amount of quantitative and qualitative data; in beetroot samples sixty-four phytochemicals have been identified therefore, this approach is recommended for more detailed metabolomics studies.
Citations
-
3 0
CrossRef
-
0
Web of Science
-
2 7
Scopus
Authors (4)
Cite as
Full text
- Publication version
- Accepted or Published Version
- License
-
open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
FOOD CHEMISTRY
no. 362,
ISSN: 0308-8146 - Language:
- English
- Publication year:
- 2021
- Bibliographic description:
- Kusznierewicz B., Konderak M., Koss-Mikołajczyk I., Namieśnik J.: Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains - Verification of beetroot samples// FOOD CHEMISTRY -Vol. 362, (2021), s.130132-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.foodchem.2021.130132
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
seen 141 times
Recommended for you
The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides
- A. Białkowska,
- J. Krysiak,
- T. Florczak
- + 3 authors