Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries

Tomasz Dymerski; Jacek Namieśnik; Kann Vearasilp; Patricia Arancibia-Avila; Fernando Toledo; Elena Katrich; Shela Gorinstein

doi:10.1016/j.talanta.2014.11.061

Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries

Abstract

The volatilefractionsofCapegooseberryandblueberryweredeterminedbyheadspacesolid-phase microextractioncoupledwithcomprehensivetwo-dimensionalgaschromatographywithtime-of-flight mass spectrometry(HS-SPME/GCGC-TOFMS).Thehighestamountofalcohol(51.8%),ester(32.8%)and carboxylicacid(6.9%)wasinblueberryincomparisonwithgooseberryandoppositelyketones(14.7%), aldehydes(9.9%)andterpenes(8%)werefoundingooseberry.Thebioactivecompoundsandantioxidant capacities werehigherinblueberriesthaningooseberries.Threedimensional fluorescenceemission spectrometry (3D-FL)wasappliedtodetermineandtocompareexperimentallyfoundbinding parametersofberriesextractswithhumanserumalbumin(HSA).The fluorescence quenchingofHSA by polyphenolsfromberrieswasaresultoftheformationofapolyphenol–HSA complex.Thebinding abilities ofberrieswerehighlycorrelatedwiththebioactivityofpolyphenolsandvolatilesubstances. The clusteranalysis(CA)andlineardiscriminantanalysis(LDA)wasappliedtodifferentiatetheberries samples accordingtotheirtype.

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Authors (7)

Tomasz Dymerski dr inż.
Jacek Namieśnik prof. dr hab. inż.
Kann Vearasilp
- Faculty of Pharmacy Srinakharinwirot University
Patricia Arancibia-Avila
- Department of Basic Sciences Universidad del Bio-Bio
Fernando Toledo
- Department of Basic Sciences Universidad del Bio-Bio
Elena Katrich
- The Institute for Drug research School of Pharmacy
Shela Gorinstein
- The Institute for Drug research School of Pharmacy

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: TALANTA.The International Journal of Pure and Applied Analytical Chemistry no. 134, pages 460 - 467,
ISSN: 0039-9140
Language:: English
Publication year:: 2015
Bibliographic description:: Dymerski T., Namieśnik J., Vearasilp K., Arancibia-Avila P., Toledo F., Katrich E., Gorinstein S.: Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries// TALANTA. -Vol. 134, (2015), s.460-467
DOI:: Digital Object Identifier (open in new tab) 10.1016/j.talanta.2014.11.061
Verified by:: Gdańsk University of Technology