DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method

Patrycja Jakubek; Jovana Rajić; Monika Baranowska; Melita Vidaković; Agnieszka Bartoszek-Pączkowska; Jacek Namieśnik

doi:10.3390/proceedings2019011020

DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method

Abstract

The impact of catechins on the expression profile of redox-related genes in HT29 cell line has been studied recently by our group using Oxidative Stress RT2 Profiler PCR Array. Within the examined panel of 84 genes, the down-regulation of SRXN1 gene was unique among other up-regulated genes. We hypothesized that the observed down-regulation resulted from DNA methylation and have exploited this observation to choose the proper strategy to monitor the changes in DNA methylation patterns incurred by dietary antioxidants. The current study verified two PCR-based approaches.

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Authors (6)

Patrycja Jakubek
Jovana Rajić
- University of Belgrade Institute for Biological Research
Monika Baranowska
Melita Vidaković PhD
- University of Belgrade Institute for Biological Research
Agnieszka Bartoszek-Pączkowska prof. dr hab. inż.
Jacek Namieśnik prof. dr hab. inż.

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Articles

Type:

publikacja w in. zagranicznym czasopiśmie naukowym (tylko język obcy)

Published in:

Proceedings no. 11, pages 1 - 4,
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Language:

English

Publication year:

2019

Bibliographic description:

Jakubek P., Rajić J., Baranowska M., Vidaković M., Bartoszek-Pączkowska A., Namieśnik J.. DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method. Proceedings, 2019, Vol. 11, iss. 1, s.1-4

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