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Abstract
The aim of the study was to compare the discriminatory power of RAPD-PCR method with using RSD10 primer and microsatellite analysis with using (GACA)4 primer for genotyping clinical isolates of fluconazole-resistant C. albicans. Isolates were received from patients of Children's Memorial Health Institute in Warsaw. However in the case of both tested methods low number of amplified fragments was generated, nine and six different genotypes were distinguished from microsatellite analysis and RAPD-PCR respectively. An evident increase in the number of generated amplicons was achieved when PCR amplification was performed with both primers simultaneously. Surprisingly, such increase did not resulted in better discriminatory power. Only six different genotypes were distinguished, and 31 out of 44 (70%) strains tested were classified as one genotype.
Authors (5)
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Katarzyna Dzierżanowska-Fangrat
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- Category:
- Conference activity
- Type:
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Title of issue:
- Diagmol 2013 XIV Conference on Molecular Biology in Diagnostics of Infectious Diseases and Biotechnology strony 133 - 136
- Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Szweda P., Gucwa K., Romanowska E., Dzierżanowska-Fangrat K., Milewski S.: Genotyping of clinical isolates of fluconazole-resistant Candida albicans// Diagmol 2013 XIV Conference on Molecular Biology in Diagnostics of Infectious Diseases and Biotechnology/ ed. M. Niemiałtowski Warsawa: , 2013, s.133-136
- Verified by:
- Gdańsk University of Technology
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