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Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment

Abstract

2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) and ethylenediaminetetraacetic acid (EDTA) are key components of biological buffers and are frequently used as DNA stabilizers in irradiation studies. Such surface or liquid phase studies are done with the aim to understand the fundamental mechanisms of DNA radiation damage and to improve cancer radiotherapy. When ionizing radiation is used, abundant secondary electrons are formed during the irradiation process, which are able to attach to the molecular compounds present on the surface. In the present study we experimentally investigate low energy electron attachment to TRIS and methyliminodiacetic acid (MIDA), an analogue of EDTA, supported by quantum chemical calculations. The most prominent dissociation channel for TRIS is through hydroperoxyl radical formation, whereas the dissociation of MIDA results in the formation of formic and acetic acid. These compounds are well-known to cause DNA modifications, like strand breaks. The present results indicate that buffer compounds may not have an exclusive protecting effect on DNA as suggested previously.

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Authors (8)

  • Photo of  Anita Ribar

    Anita Ribar

    • Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
  • Photo of  Stefan Huber

    Stefan Huber

    • Leopold Franzens University of Innsbruck Institute for Basic Sciences in Engineering Science,
  • Photo of  Katrin Tanzer

    Katrin Tanzer

    • Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
  • Photo of  Michael Neustetter

    Michael Neustetter

    • Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
  • Photo of  Robin Schürmann

    Robin Schürmann

    • Institute of Chemistry-Physical Chemistry University of Potsdam
  • Photo of  Ilko Bald

    Ilko Bald

    • Institute of Chemistry-Physical Chemistry University of Potsdam
  • Photo of  Stephan Denifl

    Stephan Denifl

    • Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
PHYSICAL CHEMISTRY CHEMICAL PHYSICS no. 20, edition 8, pages 5578 - 5585,
ISSN: 1463-9076
Language:
English
Publication year:
2018
Bibliographic description:
Ribar A., Huber S., Śmiałek-Telega M., Tanzer K., Neustetter M., Schürmann R., Bald I., Denifl S.: Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment// PHYSICAL CHEMISTRY CHEMICAL PHYSICS. -Vol. 20, iss. 8 (2018), s.5578-5585
DOI:
Digital Object Identifier (open in new tab) 10.1039/c7cp07697e
Verified by:
Gdańsk University of Technology

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