Search
Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment
Abstract
2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) and ethylenediaminetetraacetic acid (EDTA) are key components of biological buffers and are frequently used as DNA stabilizers in irradiation studies. Such surface or liquid phase studies are done with the aim to understand the fundamental mechanisms of DNA radiation damage and to improve cancer radiotherapy. When ionizing radiation is used, abundant secondary electrons are formed during the irradiation process, which are able to attach to the molecular compounds present on the surface. In the present study we experimentally investigate low energy electron attachment to TRIS and methyliminodiacetic acid (MIDA), an analogue of EDTA, supported by quantum chemical calculations. The most prominent dissociation channel for TRIS is through hydroperoxyl radical formation, whereas the dissociation of MIDA results in the formation of formic and acetic acid. These compounds are well-known to cause DNA modifications, like strand breaks. The present results indicate that buffer compounds may not have an exclusive protecting effect on DNA as suggested previously.
Citations
-
9
CrossRef
-
0
Web of Science
-
9
Scopus
Authors (8)
-
Anita Ribar
- Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
-
Stefan Huber
- Leopold Franzens University of Innsbruck Institute for Basic Sciences in Engineering Science,
-
Katrin Tanzer
- Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
-
Michael Neustetter
- Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
-
Robin Schürmann
- Institute of Chemistry-Physical Chemistry University of Potsdam
-
Ilko Bald
- Institute of Chemistry-Physical Chemistry University of Potsdam
-
Stephan Denifl
- Leopold Franzens University of Innsbruck Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck
Cite as
Full text
full text is not available in portal
Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
no. 20,
edition 8,
pages 5578 - 5585,
ISSN: 1463-9076 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Ribar A., Huber S., Śmiałek-Telega M., Tanzer K., Neustetter M., Schürmann R., Bald I., Denifl S.: Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment// PHYSICAL CHEMISTRY CHEMICAL PHYSICS. -Vol. 20, iss. 8 (2018), s.5578-5585
- DOI:
- Digital Object Identifier (open in new tab) 10.1039/c7cp07697e
- Verified by:
- Gdańsk University of Technology
seen 216 times
Recommended for you
Electron ionization and low energy electron attachment to molecules of biological interest
- M. Śmiałek-Telega,
- K. Tanzer,
- M. Neustetter
- + 4 authors
Electron ionization and low energy electron attachment to molecules of biological interest
- M. Śmiałek-Telega,
- K. Tanzer,
- M. Neustetter
- + 4 authors
VUV irradiation studies of plasmid DNA in aqueous solution
- M. Śmialek,
- S. Hoffmann,
- M. Folkard
- + 4 authors