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In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits

Abstract

Many polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The presence of polyphenols (flavonoids and phenolic acids) in the investigated samples and their interaction with human serum albumin (HSA) was studied by HPLC, Fourier Transform Infrared (FT-IR) and three dimensional fluorescence spectroscopy (3D-FL). The highest levels of polyphenols, antioxidant and binding capacities were found in red and blond grapefruits (citrus group), followed by strawberries and apples (traditional group) and mangosteen and kiwi fruit (exotic fruit), which also contained the highest levels of protocatechuic, p-coumaric, ferulic acids and quercetin. In conclusion, for the first time, the interaction of the polyphenols with human serum albumin was evaluated by fluorometry/FTIR. The obtained binding profiles allowed the comparison of three different groups of fruits. A mixture of these fruits can be recommended for consumption.

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Authors (10)

  • Photo of  Yong-Seo Park

    Yong-Seo Park

    • Mokpo National University Department of Horticultural Science
  • Photo of  Milena Cvikrová

    Milena Cvikrová

    • Academy of Sciences of the Czech Republic Institute of Experimental Botany
  • Photo of  Olga Martincová

    Olga Martincová

    • Academy of Sciences of the Czech Republic Institute of Experimental Botany
  • Photo of  Kyung-Sik Ham

    Kyung-Sik Ham

    • Mokpo National University Department of Food Engineering
  • Photo of  Seong-Gook Kang

    Seong-Gook Kang

    • Mokpo National University Department of Food Engineering
  • Photo of  Yang-Kyun Park

    Yang-Kyun Park

    • Mokpo National University Department of Food Engineering
  • Photo of  Adamo Rombolà

    Adamo Rombolà

    • University of Bologna Department of Agricultural Sciences
  • Photo of  Zenon Jastrzebski

    Zenon Jastrzebski

    • National Medicines Institute Departemtn of Pharmacology
  • Photo of  Shela Gorinstein

    Shela Gorinstein

    • Hebraw University Institute for Drug Research

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
FOOD RESEARCH INTERNATIONAL no. 74, pages 37 - 47,
ISSN: 0963-9969
Language:
English
Publication year:
2015
Bibliographic description:
Park Y., Cvikrová M., Martincová O., Ham K., Kang S., Park Y., Namieśnik J., Rombolà A., Jastrzebski Z., Gorinstein S.: In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits// FOOD RESEARCH INTERNATIONAL. -Vol. 74, (2015), s.37-47
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.foodres.2015.04.021
Verified by:
Gdańsk University of Technology

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