Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor

Christina Tam; Bruno Collinet; Gary Lau; Satish Raina; Dominique Missiakas

doi:10.1074/jbc.m202881200

Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor

Abstract

Eo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding site for promoter DNA. Mutations that reduce transcription of Esigma(E) RNA polymerase map to sigma(E) residues 178, 181, and 183. Variant sigma(E) proteins with amino acid substitutions at residues 178, 181, or 183 do not associate with RseA. A regulatory mechanism is proposed whereby RseA binds to a C-terminal peptide of a-E and inhibits the transcription of Esigma(E) RNA polymerase by blocking promoter recognition.

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Authors (5)

Christina Tam
Bruno Collinet
Gary Lau
Satish Raina prof. dr
Dominique Missiakas

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DOI:: Digital Object Identifier (open in new tab) 10.1074/jbc.M202881200
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Category:: Articles
Type:: artykuły w czasopismach
Published in:: JOURNAL OF BIOLOGICAL CHEMISTRY no. 277, pages 27282 - 27287,
ISSN: 0021-9258
Language:: English
Publication year:: 2002
Bibliographic description:: Tam C., Collinet B., Lau G., Raina S., Missiakas D.: Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor// JOURNAL OF BIOLOGICAL CHEMISTRY -Vol. 277,iss. 30 (2002), s.27282-27287
DOI:: Digital Object Identifier (open in new tab) 10.1074/jbc.m202881200
Verified by:: Gdańsk University of Technology