Search
Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis
Abstract
A collection of 206 Staphylococcus spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group included Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus strains recovered from bovine mastitis (n = 158) and human skin wounds and soft tissues infections (n = 48). Production of substances with antimicrobial activity was observed in six strains. Five of them were recovered from bovine mastitis, and one was isolated from the infected human skin wound. Three of the six antimicrobials produced by the different strains showed substantial loss of antimicrobial activity upon treatment with proteolytic enzymes, which suggests their peptidic structure. Additional studies have shown that one of the putative bacteriocins was efficiently secreted to the liquid medium, facilitating its large-scale production and isolation. The peptide produced by the M2B strain exhibited promising activity; however, against narrow spectrum of Staphylococcus spp. clinical and animal isolates. Growth inhibition was observed only in the case of 13 (including nine S. aureus, three S. xylosus and one S. epidermidis strains) out of 206 strains tested. Important advantage of the produced agent was its high thermal stability. Fifteen minutes of incubation at 90°C did not affect its antimicrobial potential. The highest efficiency of production of the agent was demonstrated in TSB medium after 24 hours at 37°C. The researches revealed that ability to production of bacteriocin among staphylococci is not very common. Only one (S. xylosus strain assigned as M2B) out of 206 strains tested produced satisfactory amounts of antistaphylococcal bacteriocin. In spite of that, we would encourage other researchers for investigation of their collections of Staphylococcus spp. isolates towards selection strains producing antimicrobial agents.
Citations
-
4
CrossRef
-
0
Web of Science
-
4
Scopus
Authors (5)
-
John Churey
- Cornell University
-
Randy Worobo
- Cornell University
Cite as
Full text
- Publication version
- Accepted or Published Version
- License
-
open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
Polish Journal of Microbiology
no. 67,
edition 2,
pages 163 - 169,
ISSN: 1733-1331 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Magdalena Z., Churey J., Worobo R., Milewski S., Szweda P.: Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis// Polish Journal of Microbiology. -Vol. 67, iss. 2 (2018), s.163-169
- DOI:
- Digital Object Identifier (open in new tab) 10.21307/pjm-2018-018
- Sources of funding:
-
- 2012/07/N/NZ9/00939
- Verified by:
- Gdańsk University of Technology
seen 158 times
Recommended for you
Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin
- D. Dębowski,
- R. Łukajtis,
- A. Łęgowska
- + 7 authors
Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials
- K. Pełka,
- R. Worobo,
- J. Walkusz
- + 1 authors
Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
- V. Zvarych,
- M. Stasevych,
- V. Novikov
- + 5 authors