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Loop-mediated isothermal amplification (LAMP) as a diagnostic tool in detection of infectious diseases
Abstract
Loop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six to eight distinct sequences on the target DNA. The amplification is performed by DNA polymerase, which has strand-displacing activity. The whole reaction occurs in a single tube and is divided into two steps: non-cyclic and cyclic. The final products are artificial stem loop DNA structures flanking the target sequences.
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Details
- Category:
- Monographic publication
- Type:
- rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
- Title of issue:
- PhD Interdisciplinary Journal. No. 1 strony 19 - 23
- Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Drapała D., Kordalewska M.: Loop-mediated isothermal amplification (LAMP) as a diagnostic tool in detection of infectious diseases// PhD Interdisciplinary Journal. No. 1/ Sopot: GUT, 2013, s.19-23
- Verified by:
- Gdańsk University of Technology
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