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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
Abstract
Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
Polish Journal of Microbiology
no. 62,
pages 81 - 84,
ISSN: 1733-1331 - Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Olchawa A., Krawczyk B., Brillowska-Dąbrowska A.: New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique// Polish Journal of Microbiology. -Vol. 62, nr. 1 (2013), s.81-84
- Verified by:
- Gdańsk University of Technology
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