Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms - Publication - Bridge of Knowledge

Search

Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms

Abstract

A significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.

Citations

  • 1 0

    CrossRef

  • 0

    Web of Science

  • 1 0

    Scopus

Cite as

Full text

download paper
downloaded 23 times
Publication version
Accepted or Published Version
DOI:
Digital Object Identifier (open in new tab) 10.18388/abp.2015_
License
Creative Commons: CC-BY-SA open in new tab

Keywords

Details

Category:
Articles
Type:
artykuły w czasopismach
Published in:
Acta Biochimica Polonica no. 63, pages 39 - 52,
ISSN: 0001-527X
Language:
English
Publication year:
2016
Bibliographic description:
Krawczyk B., Kur J., Stojowska-Swędrzyńska K., Śpibida M.: Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms// Acta Biochimica Polonica -Vol. 63,iss. 1 (2016), s.39-52
DOI:
Digital Object Identifier (open in new tab) 10.18388/abp.2015_1192
Verified by:
Gdańsk University of Technology

seen 130 times

Recommended for you

Meta Tags