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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms

Abstrakt

A significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.

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Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
Acta Biochimica Polonica nr 63, strony 39 - 52,
ISSN: 0001-527X
Język:
angielski
Rok wydania:
2016
Opis bibliograficzny:
Krawczyk B., Kur J., Stojowska-Swędrzyńska K., Śpibida M.: Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms// Acta Biochimica Polonica. -Vol. 63, iss. 1 (2016), s.39-52
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.18388/abp.2015_
Weryfikacja:
Politechnika Gdańska

wyświetlono 68 razy

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