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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
Abstract
Purpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available databases. Thus, there is a clear need for the development of new molecular methods that would provide simple, rapid and highly specific identification of Scopulariopsis and Microascus species. The objective of this study was to develop simple and fast assays based on PCR and real-time PCR for specific detection of fungi from Scopulariopsis and Microascus genera, and separately, S. brevicaulis species.
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Authors (3)
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Tomasz Jagielski dr
- University of Warsaw Department of Applied Microbiology, Faculty of Biology, Institute of Microbiology
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- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
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MYCOPATHOLOGIA
pages 465 - 474,
ISSN: 0301-486X - Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Kordalewska M., Jagielski T., Brillowska-Dąbrowska A.: Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species// MYCOPATHOLOGIA. -, nr. 181 (2016), s.465-474
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
- Verified by:
- Gdańsk University of Technology
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