Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species - Publication - Bridge of Knowledge

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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species

Abstract

Purpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available databases. Thus, there is a clear need for the development of new molecular methods that would provide simple, rapid and highly specific identification of Scopulariopsis and Microascus species. The objective of this study was to develop simple and fast assays based on PCR and real-time PCR for specific detection of fungi from Scopulariopsis and Microascus genera, and separately, S. brevicaulis species.

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DOI:
Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
MYCOPATHOLOGIA pages 465 - 474,
ISSN: 0301-486X
Language:
English
Publication year:
2016
Bibliographic description:
Kordalewska M., Jagielski T., Brillowska-Dąbrowska A.: Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species// MYCOPATHOLOGIA. -, nr. 181 (2016), s.465-474
DOI:
Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
Verified by:
Gdańsk University of Technology

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