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TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp.,recognizes novel asymmetric sequence 5´-ACGGA(N11/9)-3
Abstract
A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found in Thermus sp. GW cells.
Authors (4)
-
Agnieszka Żylicz-Stachula
-
Renata I. Harasimowicz-Słowińska
-
Ireneusz Sobolewski
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- Copyright (2002 Oxford University Press)
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie z listy filadelfijskiej
- Published in:
-
NUCLEIC ACIDS RESEARCH
no. 30,
edition 7 e33,
pages 1 - 4,
ISSN: 0305-1048 - Language:
- English
- Publication year:
- 2002
- Bibliographic description:
- Żylicz-Stachula A., Harasimowicz-Słowińska R., Sobolewski I., Skowron P.: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp.,recognizes novel asymmetric sequence 5´-ACGGA(N11/9)-3// NUCLEIC ACIDS RESEARCH. -Vol. 30., iss. 7 e33 (2002), s.1-4
- Verified by:
- Gdańsk University of Technology
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