Search results for: B-GALACTOSIDASE

• PHYSICAL REVIEW B

  Journals

  ISSN: 2469-9950 , eISSN: 2469-9969

• JOURNAL OF PHYSICAL CHEMISTRY B

  Journals

  ISSN: 1520-6106 , eISSN: 1520-5207

• B-GALACTOSIDASE ARTHROBACTER SP. 32cB - OBTAINING THE GENE SEQUENCE, CONSTRUCTION OF THE EXPRESSION SYSTEM, BIOSYNTHESIS AND BIOCHEMICAL CHARACTERIZATION OF THE ENZYME

  Publication

  • A. Pawlak-Szukalska
  • M. Wanarska
  • J. Kur
  • A. Popinigis

  - Postępy Mikrobiologii - Year 2013

  INTRODUCTION: β-Galactosidase is an enzyme which catalyzes the hydrolysis of O glycosidic bond in β-galactosides. Another activity of β galactosidase is a transglycosylation activity. The main industrial use of this protein is the hydrolysis of lactose in milk in a cooling conditions. Synthesis of galactooligosaccharides, which are mostly used as a prebiotics added to some foods or available as dietary supplements, is only one...

• SENSORS AND ACTUATORS B-CHEMICAL

  Journals

  ISSN: 0925-4005

• A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.

  Publication

  • A. Białkowska
  • H. Cieśliński
  • K. Nowakowska
  • J. Kur
  • M. Turkiewicz

  - ARCHIVES OF MICROBIOLOGY - Year 2009

  A psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...

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• APPLIED CATALYSIS B-ENVIRONMENTAL

  Journals

  ISSN: 0926-3373 , eISSN: 1873-3883

• Molecular modelling of a psychrophilic β-galactosidase

  Publication

  • K. Tkaczuk
  • J. M. Bujnicki
  • A. Białkowska
  • S. Bielecki
  • M. Turkiewicz
  • H. Cieśliński
  • J. Kur

  - Year 2005

  Antarktyczny szczep, gram-ujemnych bakterii, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, sklasyfikowany na podstawie analizy sekwencji nukleotydowej geny 16S rDNA jako Pseudoalteromonas sp. 22b jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Ponieważ enzym ten wykazuje wysoką aktywność katalityczną w zakresie temperatur od 0 do 30 stC, stanowi on atrakcyjny biokatalizator, użyteczny...

• Beta-galactosidase activity of Meiothermus ruber cells

  Publication

  • J. Synowiecki
  • I. Sinkiewicz
  • A. Zakrzewska
  • K. Grubiak
  • O. Pietrow-Tobiszewska

  - JOURNAL OF FOOD BIOCHEMISTRY - Year 2011

  Freeze-dried cells of Meiothermus ruber catalyses cleavage of o-nitrophenyl-b-D-galactopiranoside (oNPb-gal) and conversion of lactose into glucose and galactose. The permeabilization with 2%toluene,20%ethanol and 20%acetone increased enzymatic activity from 74.87 U/g of lyophilized cells up to 129.44,114.38 and 90.19 U/g,respectively. Ethanol was an effective permeabilizing agent and its efficiency was dependent on the concentration,...

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• COMPOSITES PART B-ENGINEERING

  Journals

  ISSN: 1359-8368 , eISSN: 1879-1069

• A bifunctional chimeric protein consisting of MutS and beta-galactosidase

  Publication

  • P. Sachadyn
  • A. Stanisławska-Sachadyn
  • J. Kur

  - Journal of Biotechnology - Year 2007

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