Wyniki wyszukiwania dla: MSRE-QPCR

• A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction

  Publikacja

  • M. Krygier
  • J. Podolak-Popinigis
  • J. Limon
  • P. Sachadyn
  • A. Stanisławska-Sachadyn

  - ANALYTICAL BIOCHEMISTRY - Rok 2016

  DNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...

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