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Description
The goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the tested genes from the C. albicans SC5314 genome using the SAT1-flipper method.
The deletion of tested genes encoding enzymes O-acetylhomoserine sulfhydrylases Met15p, homoserine O-acetyltransferases Met2p and cystathionine-γ-synthases Str2p enable the assessment of the impact of those enzymes on the phenotype of cells, on the virulence of the strain, and allows to recognize the in vivo function of the tested proteins in L-methionine biosynthesis.
The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
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CC BYAttribution - Raw data:
- Data contained in dataset was not processed.
Details
- Year of publication:
- 2023
- Verification date:
- 2023-03-30
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/xak8-rr65 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
- dataset L-Homoserine O-acetyltransferase (CaMet2p) activity and inhibition
- dataset Overproduction of CaMet15p native and His-tag versions.
- dataset Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
- dataset Antifungal activity of L-homoserine O-acetyltransferase (CaMet2p) inhibitors.
- dataset Overproduction of CaStr2p native and His-tag versions.
- dataset Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
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