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Description
The goal of the study was to obtain plasmid containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (STR2) and to introduce a cleavage site for restriction enzymes into them.
The constructed cassettes will enable the removal of the tested genes from the C. albicans SC5314 genome using the SAT1-flipper method.
The deletion of tested genes encoding cystathionine-γ- synthase STR2p enable the assessment of the impact of this enzyme on the phenotype of cells, on the virulence of the strain, and allows to recognize the in vivo function of the tested proteins in L-methionine biosynthesis.
The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
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CC BYAttribution
Details
- Year of publication:
- 2025
- Verification date:
- 2025-02-19
- Creation date:
- 2025
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- biological sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/d7j5-3894 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
- dataset L-Homoserine O-acetyltransferase (CaMet2p) activity and inhibition
- dataset Overproduction of CaMet15p native and His-tag versions.
- dataset Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
- dataset Antifungal activity of L-homoserine O-acetyltransferase (CaMet2p) inhibitors.
- dataset Inserts amplification for knockout cassette construction
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