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Description
The dataset contains the description of methodology and data files with the results obtained spectrophotometrically at 412 nm to determine the rate of fungal enzyme activity. The goal of the study was to obtain conditions optimal for L-homoserine O-acetyltransferase (Met2p) activity as well as evaluation of inhibitory potential of commercially available and synthesized within the frame of the project substrates analogues.
The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).
Dataset file
CaMet2p activity and inhibition.zip
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00a7418ae6aa6ea7f75ad1c7069506d9-1,
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File details
- License:
-
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CC BYAttribution - Raw data:
- Data contained in dataset was not processed.
Details
- Year of publication:
- 2022
- Verification date:
- 2022-07-19
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/x3k3-yy19 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset Antifungal activity of L-homoserine O-acetyltransferase (CaMet2p) inhibitors.
- dataset Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
- dataset Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
- dataset Overproduction of CaStr2p native and His-tag versions.
- dataset Assay of Cystathionine-γ-synthase (CaStr2p) activity determination.
- dataset Inserts amplification for knockout cassette construction
- dataset Overproduction of CaMet15p native and His-tag versions.
- dataset Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
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