Year 2024

• Application of human bile salts for in vitro digestion models

  Publication

  • D. Dulko

  - Year 2024

  In this study, experimental in vitro models simulating the environment of the human gastrointestinal tract were used to assess the impact of physiological surfactants, such as bile salts, on the kinetics of digestion. Bile salts are biosurfactants synthesised in the liver and secreted together with bile into the small intestine. There are many reports on the role of bile salts in lipolysis, but the knowledge of their influence...

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Year 2022

• Analysis of the Factors Affecting Static In Vitro Pepsinolysis of Food Proteins

  Publication

  • N. Maeda
  • D. Dulko
  • A. Macierzanka
  • C. Jungnickel

  - MOLECULES - Year 2022

  In this meta-analysis, we collected 58 publications spanning the last seven decades that reported static in vitro protein gastric digestion results. A number of descriptors of the pepsinolysis process were extracted, including protein type; pepsin activity and concentration; protein concentration; pH; additives; protein form (e.g., ‘native’, ‘emulsion’, ‘gel’, etc.); molecular weight of the protein; treatment; temperature; and...

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Year 2021

• Importance of Bile Composition for Diagnosis of Biliary Obstructions

  Publication

  • Ł. Krupa
  • R. Staroń
  • D. Dulko
  • N. Łozińska
  • A. Mackie
  • N. Rigby
  • A. Macierzanka
  • A. Markiewicz
  • C. Jungnickel

  - MOLECULES - Year 2021

  Determination of the cause of a biliary obstruction is often inconclusive from serum analysis alone without further clinical tests. To this end, serum markers as well as the composition of bile of 74 patients with biliary obstructions were determined to improve the diagnoses. The samples were collected from the patients during an endoscopic retrograde cholangiopancreatography (ERCP). The concentration of eight bile salts, specifically...

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• The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin

  Publication

  • D. Dulko
  • R. Staroń
  • L. Krupa
  • N. Rigby
  • A. Mackie
  • K. Gutkowski
  • A. Wasik
  • A. Macierzanka

  - FOOD RESEARCH INTERNATIONAL - Year 2021

  The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist in intestinal lipolysis. However, human bile can potentially influence proteins that are largely resistant to gastric digestion, and which are mainly hydrolysed...

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Year 2019

• Which casein in sodium caseinate is most resistant to in vitro digestion? Effect of emulsification and enzymatic structuring

  Publication

  • B. Franziska
  • D. Didier
  • D. Dulko
  • B. Balazs
  • A. Mackie
  • A. Macierzanka

  - FOOD HYDROCOLLOIDS - Year 2019

  We investigated the resistance of individual constituent casein epitopes (αS1-, αS2-, β- and κ-CN) in food-grademilk protein sodium caseinate (NaCN) to simulated human gastro-duodenal digestion. The influence of NaCNadsorption to the surface of oil-in-water emulsion droplets and the effect of crosslinking of the protein withenzyme transglutaminase (TG) on the proteolysis were studied by indirect ELISA. TG crosslinking renderedfragments...

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Year 2017

• Badanie wpływu struktury białek pokarmowych na kinetykę ich trawienia

  Publication

  • D. Dulko

  - Year 2017

  W dzisiejszych czasach coraz więcej ludzi cierpi na choroby cywilizacyjne związane z nieprawidłowym odżywianiem, takie jak: otyłość, cukrzyca, schorzenia układu krwionośnego, czy sarkopenia. Naukowcy z całego świata starają się znaleźć rozwiązania mające na celu zapobieganie wspomnianym problemom. Jedna z metod, która w tym pomaga, wykorzystuje modele symulujące warunki fizjologiczne ludzkiego układu pokarmowego w warunkach in...

Year 2016

• APPLICATION OF MAGNETIC NANOPARTICLES FOR WATER TREATMENT

  Publication

  • D. Dulko

  - Technical Issues - Year 2016

  In this study magnetic nanoparticles were fabricated and used for water treatment. Nanoparticles were prepared in two ways. The first one involved NiZn ferrite nanoparticles synthesized by co-precipitation of metal cations with sodium hydroxide at high temperature. The second one featured maghemite nanoparticles was prepared by saltassisted solid-state reaction. Modification and functionalization of nanoparticles surface was investigated....

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• Zastosowanie nanocząstek magnetycznych do oczyszczania wody

  Publication

  • D. Dulko

  - Year 2016

  Celem pracy było wytworzenie nanocząstek magnetycznych do usuwania zanieczyszczeń z wody. Wytworzono je dwoma sposobami. Pierwszym z nich była synteza niklowo-cynkowych ferrytowych (Ni0.5Zn0.5Fe2O4) nanocząstek metodą współstrącenia kationów metali wodorotlenkiem sodu w podwyższonej temperaturze [Majewski 2007]. Drugim sposobem była tzw. metoda sucha, przy pomocy której otrzymano maghemit [Lu i in. 2006]. Dokonano modyfikacji oraz...