Application of ion chromatography with pulsed amperometric detection for the determination of trace cyanide in biological samples, including breast milk

Ewa Jaszczak-Wilke; Krystyna Kozioł; Bogumiła Kiełbratowska; Żaneta Polkowska

doi:10.1016/j.jchromb.2019.02.008

Application of ion chromatography with pulsed amperometric detection for the determination of trace cyanide in biological samples, including breast milk

Abstract

Cyanide (CN) is the biomarker of exposure to the components of tobacco smoke, although its presence in biological samples is also due to the consumption of products containing cyanogenic glycosides. In this work, we determine the concentration of the free cyanide in urine, saliva and breast milk matrices, using ion chromatography with pulsed amperometric detection (IC-PAD). IC-PAD is an emerging method, with only few documented applications in urine and saliva, and the presented determination in breast milk is its first published report for any method. The biological samples, which were obtained from women staying in a maternity ward, showed cyanide concentrations spanning 1.82–98.47 μg L-1. Under the optimized chromatographic conditions, the IC-PAD system exhibited satisfactory repeatability (R <3%, n=3) and good linearity in the range of 1–100 μg L-1. Thus, it proved to be an effective tool for monitoring trace cyanide concentration in a series of human body fluid matrices, including breast milk. This last matrix is especially important due to the possible effect on infant health related to the mothers’ smoking habits.

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Authors (4)

Ewa Jaszczak-Wilke
Krystyna Kozioł dr
Bogumiła Kiełbratowska dr
- Gdański Uniwersytet Medyczny Klinika Położnictwa
Żaneta Polkowska prof. dr hab. inż.

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES pages 36 - 42,
ISSN: 1570-0232
Language:: English
Publication year:: 2019
Bibliographic description:: Jaszczak E., Kozioł K., Kiełbratowska, B., Polkowska Ż.: Application of ion chromatography with pulsed amperometric detection for the determination of trace cyanide in biological samples, including breast milk// JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. -, nr. 1110-1111 (2019), s.36-42
DOI:: Digital Object Identifier (open in new tab) 10.1016/j.jchromb.2019.02.008
Verified by:: Gdańsk University of Technology