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Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry
Abstract
Detecting proteins via surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative and quantitative detection of Cytochrome C under optimized experimental conditions. Boron doped carbon nanowalls (B-CNWs) with a [B]/[C] ratio of 5000 ppm and growing time of 4 h have shown the best performance in terms of signal intensity and reliability. Then, the detection of ricin, a ribosomal-inhibiting protein (RIP) classified as category B bioterrorism agent by CDC (Centre of Disease and Control and Prevention), was performed. For the first time, direct SALDI-MS detection of ricin B chain was reported without tedious sample preparation steps or database interrogation, and results were obtained within few minutes and a limit of detection (LOD) of 0.5 pmol/μl was obtained. Thanks to the introduction of galactosamine residues on B–CNW, we were able to selectively detect ricin B chain protein in complex media such as serum and soft drinks with enhanced signal intensity. B-CNWs are not toxic and are adaptable to any commercial MALDI-TOF mass spectrometer, showing their great potential as SALDI based materials.
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Authors (5)
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Ioana Hosu
- University of Lille
-
Yannick Coffinier
- University of Lille
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
TALANTA.The International Journal of Pure and Applied Analytical Chemistry
no. 252,
ISSN: 0039-9140 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Hosu I., Sobaszek M., Ficek M., Bogdanowicz R., Coffinier Y.: Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry// TALANTA.The International Journal of Pure and Applied Analytical Chemistry -Vol. 252, (2023), s.123778-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.talanta.2022.123778
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
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