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Determination of isoprostanes in exhaled breath condensate samples by liquid chromatography coupled with tandem mass spectrometry
Abstract
Oxidative stress (OS) is a state of imbalance between continuously generating reactive oxygen species (ROS) during normal cell metabolism and mechanism of detoxifying oxygen radicals through a network of antioxidative enzymes. Isoprostanes are prostaglandin -like substances that are produced in vivo primarily by free radical-induced peroxidation of arachidonic acid. The measurement of isoprostanes in biological fluids and/or tissue specimens has important clinical implications. Different analytical approaches have been used for the measurement of F2-isoprostanes, including GC-MS, LC-MS/MS, and immunoassays. . Because of its versatility and broad applicability, LC-MS/MS overcomes some of the limitations of identifying and quantifying many oxidative stress markers. A fast liquid chromatography - tandem mass spectrometry method was developed for the determination of four isomers from group of F2-isoprostanes in exhaled breath sample (EBC).
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- Category:
- Conference activity
- Type:
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Title of issue:
- 14th International Symposium of Students and Young Mechanical Engineers :''Advances in Chemical and Mechanical Engineering". - Vol. 1/2, Gdansk, 5th-7th May 2011 strony 247 - 251
- Language:
- English
- Publication year:
- 2011
- Bibliographic description:
- Janicka M., Zygler A., Kot-Wasik A., Namieśnik J.: Determination of isoprostanes in exhaled breath condensate samples by liquid chromatography coupled with tandem mass spectrometry// 14th International Symposium of Students and Young Mechanical Engineers :''Advances in Chemical and Mechanical Engineering". - Vol. 1/2, Gdansk, 5th-7th May 2011/ ed. ed. C. Fijało. - Faculty Mechanical Engineering, Chemical Faculty Gdansk University of Technology. Gdańsk: , 2011, s.247-251
- Verified by:
- Gdańsk University of Technology
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