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Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis
Abstract
Isoprostanes (IsoPs) are key biomarkers for investigating the role of free radical generation in the pathogenesis of human disorders. To solve IsoPs-related problems with regard to isoprostanes, analytical tools are required. This paper reviews the problems and trends in this field focusing on the methodology for assaying biomarkers in exhaled breath condensate (EBC) samples. A large amount of work has been done in the qualitative and quantitative analysis of IsoPs, but a standardized method has yet to emerge. The methodologies described differ, either in the sample preparation steps or in the detectiontechniques, or both. Requiring a number of chromatographic steps, the relevant extraction and purification procedures are often critical and time-consuming, and they lead to a substantial loss of target compounds. Recent data show that EBC is a promising non-invasive tool for the evaluation of different diseases. Two main analytical approaches have been adopted for IsoPs measurement: immunological methods and mass spectrometry. The methodologies for the extraction, purification and analysis of IsoPs in EBC samples are presented.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
no. 11,
pages 4631 - 4659,
ISSN: 1661-6596 - Language:
- English
- Publication year:
- 2010
- Bibliographic description:
- Janicka M., Kot-Wasik A., Kot J., Namieśnik J.: Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. -Vol. 11, iss. 11 (2010), s.4631-4659
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/ijms11114631
- Sources of funding:
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- COST_FREE
- Verified by:
- Gdańsk University of Technology
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