Search
Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis
Abstract
Isoprostanes (IsoPs) are key biomarkers for investigating the role of free radical generation in the pathogenesis of human disorders. To solve IsoPs-related problems with regard to isoprostanes, analytical tools are required. This paper reviews the problems and trends in this field focusing on the methodology for assaying biomarkers in exhaled breath condensate (EBC) samples. A large amount of work has been done in the qualitative and quantitative analysis of IsoPs, but a standardized method has yet to emerge. The methodologies described differ, either in the sample preparation steps or in the detectiontechniques, or both. Requiring a number of chromatographic steps, the relevant extraction and purification procedures are often critical and time-consuming, and they lead to a substantial loss of target compounds. Recent data show that EBC is a promising non-invasive tool for the evaluation of different diseases. Two main analytical approaches have been adopted for IsoPs measurement: immunological methods and mass spectrometry. The methodologies for the extraction, purification and analysis of IsoPs in EBC samples are presented.
Citations
-
8 7
CrossRef
-
0
Web of Science
-
9 4
Scopus
Authors (4)
Cite as
Full text
- Publication version
- Accepted or Published Version
- License
-
open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
no. 11,
pages 4631 - 4659,
ISSN: 1661-6596 - Language:
- English
- Publication year:
- 2010
- Bibliographic description:
- Janicka M., Kot-Wasik A., Kot J., Namieśnik J.: Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. -Vol. 11, iss. 11 (2010), s.4631-4659
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/ijms11114631
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
seen 128 times
Recommended for you
The compositional space of exhaled breath condensate and its link to the human breath volatilome
- F. Moritz,
- M. Janicka,
- A. Zygler
- + 6 authors
Influence of Diosmin Treatment on the Level of Oxidative Stress Markers in Patients with Chronic Venous Insufficiency
- M. Feldo,
- M. Woźniak,
- M. Wójciak-Kosior
- + 6 authors