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Abstract
Staphylococcus aureus is an aggressive pathogen responsible for a variety of diseases, in cluding life-threatening sepsis. Many strains of these bacteria are resistant to multiple classes of antibiotics, which is a substantial clinical problem in the treatment of S. aureus infections. We developed a comprehensive untargeted lipidomic workflow, including sample preparation, liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF MS) analysis and data processing, designed to study the S. aureus lipidome1. An untargeted lipidomic fingerprinting-based study involving the analysis of fourteen phenotypically diver sified clinical isolates was performed to compare the lipid fingerprints of S. aureus clinical isolates that are resistant and sensitive to five antibiotics and to extract information related to their resistance to antibiotics. To date, this is the first study to show significant differences in the lipid fingerprints between antibiotics-resistant and antibiotic-sensitive S. aureus clinical isolates2. Differences in the lipid patterns between sensitive and resistant S. aureus strains suggest that antibiotic susceptibility may be associated with the lipid composition of bacte rial cells.
Authors (6)
-
Joanna Nakonieczna dr
- Międzyuczelniany Wydział Biotechnologii UG i GUMed Katedra Biotechnologii/Zakład Diagnostyki Molekularnej
-
Mariusz Belka
- Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej Katedra i Zakład Chemii Farmaceutycznej
-
Tomasz Bączek
- Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej Katedra i Zakład Chemii Farmaceutycznej
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Keywords
Details
- Category:
- Conference activity
- Type:
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Title of issue:
- 18th International Symposium on Advances in Extraction Technologies (ExTech’2016) and the 22nd International Symposium on Separation Science (ISSS’2016) strony 145 - 145
- Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Hewelt-Belka W., Nakonieczna J., Belka M., Bączek T., Namieśnik J., Kot-Wasik A.: Lipidomics of Staphylococcus aureus – a new insight into the antibiotic resistant phenotype// 18th International Symposium on Advances in Extraction Technologies (ExTech’2016) and the 22nd International Symposium on Separation Science (ISSS’2016)/ ed. B. Buszewski & J. Kowalska : , 2016, s.145-145
- Verified by:
- Gdańsk University of Technology
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