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Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate
Abstract
Cells and tissues are constantly exposed to chemical and physical signals that regulate physiological and pathological processes. This study explores the integration of two biophysical methods: traction force microscopy (TFM) and optically detected magnetic resonance (ODMR) to concurrently assess cellular traction forces and the local relative temperature. We present a novel elastic substrate with embedded nitrogen-vacancy microdiamonds that facilitate ODMR-TFM measurements. Optimization efforts focused on minimizing sample illumination and experiment duration to mitigate biological perturbations. Our hybrid ODMR-TFM technique yields TFM maps and achieves approximately 1 K precision in relative temperature measurements. Our setup employs a simple wide-field fluorescence microscope with standard components, demonstrating the feasibility of the proposed technique in life science laboratories. By elucidating the physical aspects of cellular behavior beyond the existing methods, this approach opens avenues for a deeper understanding of cellular processes and may inspire the development of diverse biomedical applications.
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Authors (8)
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Tomasz Kołodziej
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Mariusz Mrózek dr
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Saravanan Sengottuvel
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Maciej Głowacki dr inż.
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Wojciech Gawlik prof. dr hab.
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Zenon Rajfur
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Adam M. Wojciechowski dr
Cite as
Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1364/BOE.524293
- License
-
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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Biomedical Optics Express
no. 15,
pages 4024 - 4043,
ISSN: 2156-7085 - Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Kołodziej T., Mrózek M., Sengottuvel S., Głowacki M., Ficek M., Gawlik W., Rajfur Z., Wojciechowski A. M.: Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate// Biomedical Optics Express -,iss. 7 (2024), s.4024-
- DOI:
- Digital Object Identifier (open in new tab) 10.1364/boe.524293
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
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