Real-Time PCR: molecular technique of many applications

Milena Kordalewska; Dorota Drapała; Bartłomiej Ferra

Real-Time PCR: molecular technique of many applications

Abstract

Real-Time PCR is a sensitive DNA amplification technique initially applied in genetics and molecular biology. It enables in vivo copying of the selected DNA fragment (flanked by two primers) by the thermostable polymerase (in the presence of magnesium ions and deoxynucleotide triphosphates) and simultaneous measurement of the fluorescence. For one or more specific sequences in a DNA sample, real-time PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amountwhen normalized to DNA input or additional normalizing genes.An introduction of real-time PCR enables the laboratory to obtain results in a shorter time than the traditional PCR methods do. It also improves quantitative accuracy of the results. Many alternative instruments and fluorescent probe systems have been developed recently, so realtime PCR has been adopted for a wide range of new applications e.g. mutation analysis, oncological diagnostics, microbial infections discrimination. Moreover, it has started beingapplied in food industry and agriculture to identify parasites, microorganisms and genetically modified organisms (GMO), what we would like to discuss in present article.

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Category:: Monographic publication
Type:: rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
Title of issue:: ''Advances in Chemical and Mechanical Engineering''. - Volume I/II strony 269 - 274
Language:: English
Publication year:: 2012
Bibliographic description:: Kordalewska M., Drapała D., Ferra B.: Real-Time PCR: molecular technique of many applications// ''Advances in Chemical and Mechanical Engineering''. - Volume I/II/ ed. eds. C. Fijało, P. Fijało. Gdańsk: GUT, 2012, s.269-274
Verified by:: Gdańsk University of Technology