PCR-ELISA: inexpensive alternative to quantitative PCR

Dorota Drapała; Milena Kordalewska; Bartłomiej Ferra

PCR-ELISA: inexpensive alternative to quantitative PCR

Abstract

PCR-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay), a combination of PCR and ELISA methods, has been used since late 1980s. The technique is based on specially labelled DNA fragments which are captured by specific DNA sequences and detected by antibodies. The whole procedure of PCR-ELISA is divided into three steps: DNA extraction, PCR reaction and detection by ELISA. The method has been found as very specific and sensitive which allows detection of really small amounts of DNA. Moreover, ELISA step may be automated what makes this technique faster in comparison with classical detection methods. For these reasons PCR-ELISA has been applied in many laboratories in e.g. routine microbiological diagnostics.

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Category:: Monographic publication
Type:: rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
Title of issue:: PhD Interdisciplinary Journal. - No. 1 strony 7 - 12
Language:: English
Publication year:: 2012
Bibliographic description:: Drapała D., Kordalewska M., Ferra B.: PCR-ELISA: inexpensive alternative to quantitative PCR// PhD Interdisciplinary Journal. - No. 1/ ed. ed. M. Czubenko. - Gdansk University of Technology. Gdańsk: , 2012, s.7-12
Verified by:: Gdańsk University of Technology