Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva

Paweł Wityk; Agata Terebieniec; Robert Nowak; Jacek Łubiński; Martyna Mroczyńska-Szeląg; Tomasz Wityk; Dorota Kostrzewa-Nowak,

doi:10.3390/s25020360

Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva

Abstract

Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements. The biosensor is based on electrochemical principles, employing oligonucleotide probes immobilized on a hydrophobic-coated electrode, which prevents air bubbles and salt crystal formation. During validation, the biosensor demonstrated a sensitivity and specificity of 100%, accurately identifying SARS-CoV-2 in saliva samples without false positives or negatives. Cross-validation with RT-qPCR, the gold standard for COVID-19 diagnostics, confirmed the reliability of our device. The biosensor’s performance was tested on 60 participants, yielding 12 true positive results and 48 true negatives, aligning perfectly with RT-qPCR outcomes. This reusable, easy-to-use biosensor offers significant potential for point-of-care applications in various healthcare settings, providing a fast, efficient, and cost-effective method for detecting viral infections such as COVID-19. Its robust design, minimal sample preparation requirements, and multiple-use capability mark a significant advancement in biosensing technology.

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Authors (7)

Paweł Wityk dr inż.
- Map Your DNA Ltd., Świerkowa 40, 83-330 Lniska, Poland; Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Biopharmaceutics and Pharmacodynamics, Medical University of
Agata Terebieniec mgr inż.
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 CS Utrecht, The Netherlands
Robert Nowak dr hab.
- Institute of Physical Culture Sciences, University of Szczecin, 40B Piastów Al., Building 6, 71-065 Szczecin, Poland; Department of Pathology, Pomeranian Medical University in Szczecin, 1 Unii Lubelskiej St., 71-242 Szczecin, Poland
Jacek Łubiński dr hab. inż.
Martyna Mroczyńska-Szeląg dr inż.
Tomasz Wityk
- Map Your DNA Ltd., Świerkowa 40, 83-330 Lniska, Poland
Dorota Kostrzewa-Nowak, dr
- Department of Clinical and Molecular Biochemistry, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp. Al., 70-111 Szczecin, Poland

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Category:

Articles

Type:

artykuły w czasopismach

Published in:

SENSORS no. 25,
ISSN: 1424-8220

Language:

English

Publication year:

2025

Bibliographic description:

Wityk P., Terebieniec A., Nowak R., Łubiński J., Mroczyńska-Szeląg M., Wityk T., Kostrzewa-Nowak, D.: Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva// SENSORS -,iss. 2 (2025), s.360-

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