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Smartphone-based digital image colorimetry for the determination of total capsaicinoid content in chili pepper extracts
Abstract
A simple smartphone-based digital image colorimetry was proposed for the determination of total capsaicinoid content and the assessment of chili pepper pungency. The biobased solvent D-limonene was used for the first time to isolate analytes. Capsaicinoids were efficiently separated from chili pepper by solid-liquid extraction with D-limonene followed by partitioning of the analytes into the ammonium hydroxide solution to eliminate the matrix interference effect. For colorimetric detection of total capsaicinoid content, a selective chromogenic reaction was performed using Gibbs reagent (2,6-dichloroquinone-4-chloroimide). Measurements were performed using a smartphone-based setup and included image analysis with the program ImageJ. The limit of detection of the proposed procedure was 0.15 mg g-1. The intra-day repeatability did not exceed 10.0%. The inter-day repeatability was less than 16.5 %. The comparison of the smartphone-based procedure with high-performance liquid chromatography showed satisfactory results.
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
no. 315,
ISSN: 1386-1425 - Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Vakh K., Mallabaeva Z., Tobiszewski M.: Smartphone-based digital image colorimetry for the determination of total capsaicinoid content in chili pepper extracts// SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY -, (2024), s.124238-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.saa.2024.124238
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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