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Abstract
The aim of this study was the whole-genome analysis and assessment of the antimicrobial potential of bacterial isolates from honey harvested in one geographical location—the north of Poland. In total, 132 strains were derived from three honey samples, and the antimicrobial activity of CFAM (cell-free after-culture medium) was used as a criterion for strain selection and detailed genomic investigation. Two of the tested isolates (SZA14 and SZA16) were classified as Bacillus paralicheniformis, and one isolate (SZB3) as Bacillus subtilis based on their ANI and phylogenetic analysis relatedness. The isolates SZA14 and SZA16 were harvested from the same honey sample with a nucleotide identity of 98.96%. All three isolates have been found to be potential producers of different antimicrobial compounds. The secondary metabolite genome mining pipeline (antiSMASH) identified 14 gene cluster coding for non-ribosomal peptide synthetases (NRPs), polyketide synthases (PKSs), and ribosomally synthesized and post-translationally modified peptides (RiPPs) that are potential sources of novel antibacterials. The BAGEL4 analysis revealed the presence of nine putative gene clusters of interest in the isolates SZA14 and SZA16 (including the presence of six similar clusters present in both isolates, coding for the production of enterocin Nkr-5-3B, haloduracin-alpha, sonorensin, bottromycin, comX2, and lasso peptide), and four in B. subtilis isolate SZB3 (competence factor, sporulation-killing factor, subtilosin A, and sactipeptides). The outcomes of this study confirm that honey-derived Bacillus spp. strains can be considered potential producers of a broad spectrum of antimicrobial agents.
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Authors (5)
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Randy Worobo
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00253-023-12732-9
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
no. 107,
pages 6389 - 6406,
ISSN: 0175-7598 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Hafeez A., Pełka K., Buzun K., Worobo R., Szweda P.: Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey// APPLIED MICROBIOLOGY AND BIOTECHNOLOGY -,iss. 20 (2023), s.6389-6406
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00253-023-12732-9
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
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