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Wyniki wyszukiwania dla: MIT

  • Microscopy analysis of A549 and H460 cells' mitochondria after exposure to TXT2 and TXT4

    Dane Badawcze
    open access

    The data set contains live-cell imaging of A549 and H460 cells' mitochondria after exposure to TXT2 and TXT4. Cells were exposed for 6 h to the tested compounds at their IC90 concentration or to DMSO. Then, mitochondrial labelling was performed by incubating the cells with a MitoTracker Green FM probe (Thermo Fisher Scientific) and Hoechst 33342 (Thermo...

  • D-loop sequences retrieved from Canis lupus familiaris mitochondrial genome

    Dane Badawcze
    open access
    • K. Kowal
    • B. Ślaska
    • A. Tkaczyk-Wlizło

    Canine mitochondrial genome is built of 16727 bp. Non-coding control region (mtCR), called also D-loop, begins with 15458 nucleotide and ends with 16727 nucleotide. The length of this fragment is 1270 bp (Kim et al., 1998). D-loop region is responsible for replication and transcription of mitochondrial DNA. Mutations that occur within it may cause irregularity...

  • Molecular differences in mitochondrial DNA (mtDNA) genomes of dogs with malignant mammary tumours

    Dane Badawcze
    open access
    • B. Ślaska
    • K. Kowal
    • A. Tkaczyk-Wlizło
    • M. Pierzchała
    • J. Gawor

    The aim of this study was to determine molecular defects in mitochondrial DNA with the use of large-scale genome analysis in malignant canine mammary gland tumours and indicate whether these changes were linked with the carcinogenesis process. With the use of the NGS technology, we sequenced 27 samples of mtDNA isolated from blood and tumours obtained...