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• Heterogeneity of quaternary structure of glucosamine-6-phosphate deaminase from Giardia lamblia

  Publikacja

  • K. Kwiatkowska-Semrau
  • J. Czarnecka
  • M. Wojciechowski
  • S. Milewski

  - PARASITOLOGY RESEARCH - Rok 2015

  The oligoHis-tagged versions of glucosamine-6- phosphate deaminase from Giardia lamblia (GlmNagB-HisN, GlmNagB-HisC) were constructed and purified to hear homogeneity, and their kinetic and structural properties were compared to those of the wild-type enzyme (GlmNagB). Introduction of the oligoHis tag at the GlmNagB C-terminus resulted in almost complete loss of the catalytic activity, while the catalytic properties of GlmNagB-HisN...

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