Wyniki wyszukiwania dla: gene cloning

• Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli.

  Publikacja

  • B. Grzybowska
  • P. Szweda
  • J. Synowiecki

  - Rok 2004

  Gen kodujący alfa-amylazę z Pyrococcus woesei klonowano do plazmidów pET21d(+)lub pYTB2, którymi transformowano komórki Escherichia coli. Otrzymana rekombinantowa alfa amylaza wykazuje maksymalną aktywność przy pH 5.6 w temperaturze 95 stC i przejawia około 24 % początkowej aktywności nawet po 2 godz. inkubacji w 120 stC. Duża termostabilność otrzymanego preparatu świadczy o jego przydatności do enzymatycznego upłynniania skrobi.

• Direct cloning of heavy metal resistance genes from metagenomic DNA

  Publikacja

  • M. Golebiewski
  • E. Deja-Sikora
  • A. Tretyn

  - Journal of Biotechnology - Rok 2010

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• A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization

  Publikacja

  • A. Wierzbicka-Woś
  • H. Cieśliński
  • M. Wanarska
  • K. Kozłowska-Tylingo
  • P. Hildebrandt
  • J. Kur

  - Microbial Cell Factories - Rok 2011

  Beta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...

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• A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties

  Publikacja

  • M. Wanarska
  • Ł. Ługowski
  • J. Kur

  - Acta Biochimica Polonica - Rok 2010

  A psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...

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• Genes

  Czasopisma

  ISSN: 2073-4425

• GENE

  Czasopisma

  ISSN: 0378-1119 , eISSN: 1879-0038

• THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4

  Publikacja

  • E. Krajewska
  • M. Wanarska
  • J. Kur

  - Postępy Mikrobiologii - Rok 2013

  INTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...

• A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and properties

  Publikacja

  • P. Hildebrandt
  • M. Wanarska
  • J. Kur

  - BMC MICROBIOLOGY - Rok 2009

  The development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. In this article, we present a new β-D-galactosidase as a candidate to be applied in the above mentioned biotechnological...

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• A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization

  Publikacja

  • A. Pawlak-Szukalska
  • M. Wanarska
  • A. Popinigis
  • J. Kur

  - PROCESS BIOCHEMISTRY - Rok 2014

  A gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...

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• A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.

  Publikacja

  • A. Białkowska
  • H. Cieśliński
  • K. Nowakowska
  • J. Kur
  • M. Turkiewicz

  - ARCHIVES OF MICROBIOLOGY - Rok 2009

  A psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...

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