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Wyniki wyszukiwania dla: GENE CLONING
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Increased Expression of Several Collagen Genes is Associated with Drug Resistance in Ovarian Cancer Cell Lines
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Expression Changes in Fatty acid Metabolic Processrelated Genes in Porcine Oocytes During in Vitro Maturation
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Altered Expression of ESR1, ESR2, PELP1 and c-SRC Genes Is Associated with Ovarian Cancer Manifestation
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Enterococci isolated from plant-derived food - Analysis of antibiotic resistance and the occurrence of resistance genes
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Effects of flavonoids on expression of genes involved in cell cycle regulation and DNA replication in human fibroblasts
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Total impact of oxidative stress genes on cardiovascular events—a 7-year follow-up study
PublikacjaCardiovascular (CV) events are the number one cause of lifetime disability and deaths worldwide. It is well known that traditional risk factors do not fully correlate with clinical outcomes; therefore, searching for other markers that would explain CV events occurrence seems essential. Of importance, one of the main factors at the origin of CV events is oxidative stress, causing inflammation and atherosclerotic plaque instability....
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Elgold: gold standard, multi-genre dataset for named entity recognition and linking
Dane BadawczeThe dataset contains 276 multi-genre texts with marked named entities, which are linked to corresponding Wikipedia articles if available. Each entity was manually verified by at least three people, which makes the dataset a high-quality gold standard for the evaluation of named entity recognition and linking algorithms.
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Coping of Chronically-Ill Patients during the COVID-19 Pandemic: Comparison between Four Groups
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Differential and Overlapping Effects of 20,23(OH)2D3 and 1,25(OH)2D3 on Gene Expression in Human Epidermal Keratinocytes: Identification of AhR as an Alternative Receptor for 20,23(OH)2D3
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Cytokine TGFβ Gene Polymorphism in Asthma: TGF-Related SNP Analysis Enhances the Prediction of Disease Diagnosis (A Case-Control Study With Multivariable Data-Mining Model Development)
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Direct Current Atmospheric Pressure Microdischarge Generated between a Miniature Flow Helium Microjet and a Flowing Liquid Cathode
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Non-disjoint Decomposition Using r-admissibility and Graph Coloring and Its Application in Index Generation Functions Minimization
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Investigations on mixture preparation for two phase adiabatic pressure drop of R134a flowing in 5 mm diameter channel
PublikacjaThe article presents detailed two-phase adiabatic pressure drops data for refrigerant R134a. Study cases have been set for a mass flux varying from 200 to 400 kg/m2 s, at the saturation temperature of 19.4 ◦C. Obtained experimental data was compared with the available correlations from the literature for the frictional pressure drop during adiabatic flow. Influence of mixture preparation on pressure drop was investigated, for...
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The impact of cooking method on the phenolic composition, total antioxidant activity and starch digestibility of rice (Oryza sativa L.)
PublikacjaThis study investigated changes in the phenolic composition, total antioxidant activity (TAA) and starch digestibility in white and brown rice due to three different cooking procedures, and subsequent reheating of cooked rice after storage. Among the analyzed samples, brown rice showed the highest TAA and phenolic content (622.5 mg kg-1 DW). All cooking methods resulted in significant decrease of phenolic content and TAA of rice...
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Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences
PublikacjaZaproponowano metodę genotypowania bakterii rodzaju Acinetobacter, opartą o analizę sekwencji genów recA, amplifikację fragmentów genu recA i analizę profili fragmentów restrykcyjnych po cięciu produktów reakcji PCR enzymami restrykcyjnymi (recA PCR/RFLP). Profile RFLP otrzymane za pomocą enzymu Tsp509I są użyteczne w identyfikacji poszczególnych gatunków bakterii rodzaju Acinetobacter. Na podstawie analizy otrzymanych sekwencji...
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Sequences of large subunit ribosomal RNA gene, partial sequence encoding D1/D2 domain of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA fragments encoding D1/D2 domain of large subunit ribosomal RNA of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using NL1 5’ GCATATCAATAAGCGGAGGAAAAG 3’ and NL4 5’ GGTCCGTGTTTCAAGACGG 3’ primers and sequenced by Sanger method using the same primers.
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GENES TO CELLS
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Novel staphylococci nucH taxonomical marker used in identification of human-associated Staphylococcus succinus subsp. casei
PublikacjaThe aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR-RFLP-rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments...
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Pronounced cytostatic activity and bystander effect of a novel series of fluorescent tricyclic acyclovir and ganciclovir derivatives in herpes simplex virus thymidine kinase gene-transduced tumor cell lines
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Liver Cirrhosis in Chronic Hepatitis B Patients Is Associated with Genetic Variations in DNA Repair Pathway Genes
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Targeted DNA oxidation by LSD1–SMAD2/3 primes TGF-β1/ EMT genes for activation or repression
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8-Oxoguanine incision activity is impaired in lung tissues of NSCLC patients with the polymorphism of OGG1 and XRCC1 genes
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In uence of Low-Level Features Extracted from Rhythmic and Harmonic Sections on Music Genre Classi cation
PublikacjaWe present a comprehensive evaluation of the infuence of 'harmonic' and rhythmic sections contained in an audio file on automatic music genre classi cation. The study is performed using the ISMIS database composed of music files, which are represented by vectors of acoustic parameters describing low-level music features. Non-negative Matrix Factorization serves for blind separation of instrument components. Rhythmic components...
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Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant Staphylococcus aureus
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Functional Interfaces, Biological Pathways, and Regulations of Interferon-Related DNA Damage Resistance Signature (IRDS) Genes
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Effect of resveratrol analogue, DMU-212, on antioxidant status and apoptosis-related genes in rat model of hepatocarcinogenesis
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Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs
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Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach
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New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines
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Chosen single nucleotide polymorphisms (SNPs) of enamel formation genes and dental caries in a population of Polish children
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Analysis of MDR genes expression and cross-resistance in eight drug resistant ovarian cancer cell lines
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Erratum to: Effects of flavonoids on expression of genes involved in cell cycle regulation and DNA replication in human fibroblasts
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Transcriptome profiling reveals distinctive traits of retinol metabolism and neonatal parallels in the MRL/MpJ mouse
PublikacjaBackground: The MRL/MpJ mouse is a laboratory inbred strain known for regenerative abilities which are manifested by scarless closure of ear pinna punch holes. Enhanced healing responses have been reported in other organs. A remarkable feature of the strain is that the adult MRL/MpJ mouse retains several embryonic biochemical characteristics, including increased expression of stem cell markers. Results: We explored the transcriptome...
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Role of miR-15b/16–2 cluster network in endometrial cancer: An in silico pathway and prognostic analysis
PublikacjaEndometrial cancer (EC) is the second most common cancer in women. A large number of human cancers exhibit dysregulation of microRNA expression including EC. MiR-15b/16–2 is one of the best-known miRNA clusters that is expressed in many types of cancer tissues. Herein, we analyzed the expression of individual miR-15b/16–2 cluster members, its paralogues, and their target network analysis, as well as their prognostic significance...
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Stress coping strategies and their clinical correlates in patients with psychosis at various stages of illness: A case‐control study
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Coping Strategies and Psychopathological Responses Among Medical and Non-medical Professionals – a Cross-Sectional Online Survey
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Multicast Traffic Throughput Maximization through Dynamic Modulation and Coding Scheme Assignment in Wireless Sensor Networks
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Reliable OFDM Data Transmission with Pilot Tones and Error-Correction Coding in Shallow Underwater Acoustic Channel
PublikacjaThe performance of Underwater Acoustic Communication (UAC) systems are strongly related to the specific propagation conditions of the underwater channel. Horizontal, shallow-water channels are characterised by extremely disadvantageous transmission properties, due to strong multipath propagation and refraction phenomena. The paper presents the results of communication tests performed during a shallow, inland-water experiment with...
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An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography
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Spectroscopic Characterization of Miniaturized Atmospheric-Pressure dc Glow Discharge Generated in Contact with Flowing Small Size Liquid Cathode
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Inter-laboratory proficiency tests in measuring thermal insulation and evaporative resistance of clothing using the Newton-type thermal manikin
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Polymer journal bearings behaviour research under limited lubrication and cooling conditions conducted on high torque test rig
PublikacjaPolymer journal bearings are frequently used as a water-lubricated bearings of small marine units’ propeller shafts . Choice of these bearings is based on their simple construction and relatively low purchase and maintenance costs. Although properly designed and installed water lubricated bearings may well last for over a decade, rapid wear or failure of these bearings can be observed...
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Activation Energy and Inclination Magnetic Dipole Influences on Carreau Nanofluid Flowing via Cylindrical Channel with an Infinite Shearing Rate
PublikacjaThe infinite shear viscosity model of Carreau fluid characterizes the attitude of fluid flow at a very high/very low shear rate. This model has the capacity for interpretation of fluid at both extreme levels, and an inclined magnetic dipole in fluid mechanics has its valuable applications such as magnetic drug engineering, cold treatments to destroy tumors, drug targeting, bio preservation, cryosurgery, astrophysics, reaction kinetics,...
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Mirosław Andrusiewicz prof. dr hab. n. med. i n. o zdr.
OsobyPosiadane dyplomy, stopnie naukowe lub artystyczne ̶ stopień doktora habilitowanego nauk medycznych (dyscyplina biologia medyczna) z dnia 4 grudnia 2017 r. Tytuł osiągnięcia naukowego: „Analiza wybranych genów związanych z przebiegiem zmian patologicznych w komórkach wywodzących się z żeńskich wewnętrznych narządów płciowych”; Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu, Wydział Lekarski II; recenzenci: Prof....
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Sequences of internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA encoding ITS1-5.8S-ITS2 fragments of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using ITS1 5' TCCGTAGGTGAACCTGCGG 3' and ITS4 5’ TCCTCCGCTTATTGATATGC 3’ primers and sequenced by Sanger method using the same primers.
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublikacjaThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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Multilocus Analysis of Candidate Genes Involved in Neurogenic Inflammation in Pediatric Asthma and Related Phenotypes: A Case–Control Study
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Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
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New and Old Genes Associated with Primary and Established Responses to Cisplatin and Topotecan Treatment in Ovarian Cancer Cell Lines
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