Effect of dendrimer-based interlayers for enzyme immobilization on a model electrochemical sensing system for glutamate

Marcin Urbanowicz; Kamila Sadowska; Bartłomiej Lemieszek; Agnieszka Paziewska-Nowak; Anna Sołdatowska; Marek Dawgul; Dorota G. Pijanowska

doi:10.1016/j.bioelechem.2023.108407

Effect of dendrimer-based interlayers for enzyme immobilization on a model electrochemical sensing system for glutamate

Abstract

In this paper, we discuss dendrimer usage in enzyme-based electrochemical biosensors, particularly with respect to biomolecule loading on the sensing surface. A novel approach to design bioactive layers with immobilized enzymes for electrochemical biosensors using the surface plasmon resonance (SPR) method in combination with electrochemical impedance spectroscopy was presented. The gold surface was modified with linear linkers (various mercaptoalkanoic acids and aminoalkanethiols) and poly(amidoamines) dendrimers from the first- to fifth-generation. The best functionalization procedure was established by detailed SPR studies and transferred onto gold electrodes to electrochemically examine the model enzymatic reaction catalysed by glutamate oxidase. In the case of the chronoamperometric method, the determined sensitivity was 3.36±0.08 μA∙mM-1 , and the low limit of detection (LOD) was 1.52 μM. Comparing the sensitivity and LOD obtained for CV measurements, the values of these parameters were 2.5 times higher and 4 times lower, respectively, for the fourth-generation dendrimer-based biosensor and the biosensor with a linear linker. The positive impact of the dendrimer interlayer on the long-term enzyme activity was also confirmed. The research results indicate the possibility of a significant increase in the sensor response using the dendrimer itself without enriching it with electrochemical components.

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Authors (7)

Marcin Urbanowicz
Kamila Sadowska
Bartłomiej Lemieszek
Agnieszka Paziewska-Nowak
Anna Sołdatowska
Marek Dawgul
Dorota G. Pijanowska prof. dr hab.inż.

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Articles

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artykuły w czasopismach

Published in:

BIOELECTROCHEMISTRY no. 152,
ISSN: 1567-5394

Language:

English

Publication year:

2023

Bibliographic description:

Urbanowicz M., Sadowska K., Lemieszek B., Paziewska-Nowak A., Sołdatowska A., Dawgul M., Pijanowska D. G.: Effect of dendrimer-based interlayers for enzyme immobilization on a model electrochemical sensing system for glutamate// BIOELECTROCHEMISTRY -, (2023), s.108407-

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