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Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink.
Abstract
This study introduces a method for producing printable, thermosensitive bioink formulated from agarose (AG) and carbon dioxide-saturated chitosan (CS) hydrogels. The research identified medium molecular weight chitosan as optimal for bioink production, with a preferred chitosan hydrogel content of 40–60 %. Rheological analysis reveals the bioink's pseudoplastic behavior and a sol-gel phase transition between 27.0 and 31.5 °C. The MMW chitosan-based bioink showed also the most stable extrusion characteristic. The choice of chitosan for the production of bioink was also based on the assessment of the antimicrobial activity of the polymer as a function of its molecular weight and the degree of deacetylation, noting significant cell reduction rates for E. coli and S. aureus of 1.72 and 0.54 for optimal bioink composition, respectively. Cytotoxicity assessments via MTT and LDH tests confirm the bioink's safety for L929, HaCaT, and 46BR.1 N cell lines. Additionally, XTT proliferation assay proved the stimulating effect of the bioink on the proliferation of 46BR.1 N fibroblasts, comparable to that observed with Fetal Bovine Serum (FBS). FTIR spectroscopy confirms the bioink as a physical polymer blend. In conclusion, the CS/AG bioink demonstrates the promising potential for advanced spatial cell cultures in tissue engineering applications including skin regeneration.
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Authors (8)
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Katarzyna Czerwiec
- Gdański Uniwersytet Medyczny
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Milena Deptuła
- Gdański Uniwersytet Medyczny
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Michał Pikuła Prof. dr hab.
- Gdański Uniwersytet Medyczny
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.carbpol.2024.122120
- License
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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CARBOHYDRATE POLYMERS
no. 336,
ISSN: 0144-8617 - Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Banach-Kopeć A., Mania S., Tylingo R., Wawrzynowicz A., Pawłowska M., Czerwiec K., Deptuła M., Pikuła M.: Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink.// CARBOHYDRATE POLYMERS -Vol. 336, (2024), s.122120-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.carbpol.2024.122120
- Sources of funding:
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- IDUB
- Verified by:
- Gdańsk University of Technology
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