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Search results for: LIGATION-MEDIATED PCR
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Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism
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Corporate social responsibility and forward default risk mediated by financial performance and goodwill
PublicationIn today’s business environment, corporate social responsibility (CSR) has become an increasingly significant factor for firms. This study is driven by the motivation to add to the current literature by investigating the mediating elements that explain the relationship between CSR and forward default risk. In this paper, we attempt to identify the important mediators and give a more comprehensive explanation of this connection...
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Application of PCR-HRM method for microsatellite polymorphism genotyping in the LDHA gene of pigeons (Columba livia)
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Correlation between the Reactivity and Spectroscopic Properties of N-Substituted Secondary Thioamides. New Intramolecular N• •H+• •N Binding Approach and Proton Complexes Based on Thioamide Ligation
PublicationFrom a comparison of chem. shifts and wavenumbers of several secondary thioamides and amides having monocationic substituents attached to thiocarbamoyl or carbamoyl groups by a polymethylene chain, new intramol. unconventional N···H+···N H bonding effects were discovered. It is argued that the CH2-N rotation is hindered and two +H···NHMe nonequiv. protons occur in a proton spectrum of hydrochloride RCH2C(S)NH(CH2)3NHMe-HCl...
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CEACAM6 as a Novel Therapeutic Target to Boost HO-1—mediated Antioxidant Defense in COPD
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Role of Lentivirus-Mediated Overexpression of Programmed Death-Ligand 1 on Corneal Allograft Survival
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Donor–acceptor nonradiative energy transfer mediated by surface plasmons on ultrathin metallic films
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Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis.
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PELP1 and SRC kinase as important molecules in the estrogen-mediated pathway in human testis and epididymis
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Strategies for overcoming ABC-transporters-mediated multidrug resistance (MDR) of tumor cells. Review
PublicationOporność wielolekowa (MDR) nowotworów jest główną przyczyną niepowodzeń w chemoterapii przeciwnowotworowej. Jest ona wynikiem nadprodukcji w komórkach opornych transporterów białkowych z nadrodziny ABC, eksportujących cytostatyk z komórki, których działanie uniemożliwia utrzymanie terapeutycznego stężenia leku. W publikacji dokonano przeglądu różnych strategii przeciwdziałania zjawisku MDR. Strategie te dotyczą zarówno kontroli...
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Analysis of heat transfer and AuNPs-mediated photo-thermal inactivation of E. coli at varying laser powers using single-phase CFD modeling
PublicationPurpose In the wake of the COVID-19 pandemics, the demand for innovative and effective methods of bacterial inactivation has become a critical area of research, providing the impetus for this study. The purpose of this research is to analyze the AuNPs-mediated photothermal inactivation of E. coli. Gold nanoparticles irradiated by laser represent a promising technique for combating bacterial infection that combines high-tech and...
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PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans
PublicationOpracowano prostą w wykonaniu metodę genotypowania szczepów bakterii z gatunku Mycobacterium ulcerans. Polimorfizm genetyczny 32 badanych izolatów Mycobacterium ulcerans odzwierciedla ich geograficzne pochodzenie z rejonów Afryki Centralnej, Ameryki Południowej i Łacińskiej, Azji Południowo-Wschodniej, Malezji oraz Australii.
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Neuronal Nitric Oxide Synthase-Mediated Genotoxicity of 2-Methoxyestradiol in Hippocampal HT22 Cell Line
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Phyto-mediated photocatalysis: a critical review of in-depth base to reactive radical generation for erythromycin degradation
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Add‐on therapy with doxazosin in patients with hypertension influences arterial stiffness and albuterol‐mediated arterial vasodilation
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Transthyretin Stabilizers Treatment in Patients With Transthyretin-Mediated Cardiac Amyloidosis: A Systematic Review and Meta-Analysis
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PLDLA/TPU Matrix Enriched with Cyclosporine A as a Therapeutic Platform for Immune-Mediated Keratitis (IMMK) in Horses
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Supramolecular Assemblies of Hydrogen Bonded Carboxylic Acid Dimers Mediated by Phenyl-Pentafluorophenyl Stacking Interactions.
PublicationKwas pentafluorobenzoesowy tworzy heterodimery z kwasami benzoesowym i 2,4,6-trimetylobenzoesowym w wyniku oddziaływań typu pi-pi reszt fenylowych i pentafluorofenylowych. Otrzymane kokryształy wymienionych kwasów z 1,4-bisarylobutadiynami zawierały homodimery tworzone przez cząsteczki kwasów, pomiędzy którymi znajdowały się cząsteczki diarylodiacetylenów.
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Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles
PublicationSilver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components...
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Expression and intein-mediated purification of novel staphylokinase SakSTAR with reduced immunogenicity and antiplatelet and antithrombin activation
PublicationSkonstruowano nową formę stafylokinazy zawierającej na swoim C-końcu domenę pochodzącą z C-konieca ----(KOŃCA)---??? hirudyny. Wprowadzono do sekwencji stafylokinazy sekwencje RGD i DGR, odpowiedzialne za zmniejszenie agregacji płytek krwi (52% do 38%) a tym samym zwiększenie skuteczności działania tego białka jako potencjalnego leku. Otrzymany nowy wariant stafylokinazy posiadał zwiększoną aktywność fibrynolityczną, czego przejawem...
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Preparation of Pseudopeptides Building Blocks with Retro-Thioamide Bond Mediated via Thiocarbamoyl Meldrum's Acid
PublicationAn easy and efficient synthesis of pseudo tripeptide containing a thiomalonamide moiety was developed. Isothiocyanate derivatives of amino acids react smoothly with 2,2-dimethyl-1,3-dioxane-4,6-dione yielding new thiocarbamoyl Meldrum's acids. Thermal decomposition of this new Meldrum's acid derivatives generate thiocarbamoyl ketenes, which acylate amino acid esters to give pseudo tripeptides.
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Highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances specificity of simplex and multiplex PCR assays
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The Major Heat Shock Proteins, Hsp70 and Hsp90, in 2-Methoxyestradiol-Mediated Osteosarcoma Cell Death Model
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Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants
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Molecular transformation of dissolved organic matter in manganese ore-mediated constructed wetlands for fresh leachate treatment
PublicationThe organic matter (OM) and nitrogen in Fresh leachate (FL) from waste compression sites pose environmental and health risks. Even though the constructed wetland (CW) can efficiently remove these pollutants, the molecular-level transformations of dissolved OM (DOM) in FL remain uncertain. This study reports the molecular dynamics of DOM and nitrogen removal during FL treatment in CWs. Two lab-scale vertical-flow CW systems were...
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Correction to “Emerging Anticancer Activity of Candidal Glucosamine-6-Phosphate Synthase Inhibitors upon Nanoparticle-Mediated Delivery”
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The Effects of Acute and Chronic Haloperidol Treatment on Dopamine Release Mediated by the Medial Forebrain Bundle in the Striatum and Nucleus Accumbens
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Magnesiate-Utilized/Benzyne-Mediated Approach to Indenopyridones from 2-Pyridones: An Attempt To Synthesize the Indenopyridine Core of Haouamine
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ClpP/ClpX-mediated degradation of the bacteriophage λ O protein and regulation of λ phage and λ plasmid replication
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Comparison of siRNA-mediated silencing of glycosaminoglycan synthesis genes and enzyme replacement therapy for mucopolysaccharidosis in cell culture studies.
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In silico studies and β-cyclodextrin mediated neutral synthesis of 4-oxo-4,5,6,7-tetrahydroindoles of potential biological interest
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Diphosphination of CO2 and CS2 mediated by frustrated Lewis pairs - catalytic route to phosphanyl derivatives of formic and dithioformic acid
PublicationThe first example of CO2 diphosphination is described. Reactions of unsymmetrical diphosphanes with CE2 (E = O, S) catalyzed by BPh3 insert a CE2 molecule into the P-P bond with formation of the products of the general formula R2P-E-C(=E)-PR2. The obtained CO2 adducts arise from synergistic interaction of diphosphane and borane with CO2 molecule.
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Multiplex PCR assay for the simultaneous identification of race specific and non-specific leaf resistance genes in wheat (Triticum aestivum L.)
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Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR
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Enterococci from ready-to-eat food - horizontal gene transfer of antibiotic resistance genes and genotypic characterization by PCR melting profile
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One-tube cell lysis and DNA extraction procedure for PCR based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish.
PublicationMycobacterium ulcerans jest bakterią wywołującą choroby skóry u ludzi i zwierząt. W trakcie prowadzonych badań opracowano prostą w wykonaniu metodę ekstrakcji DNA Mycobacterium ulcerans z próbek środowiskowych oraz klinicznych. Opracowana metoda izolacji DNA może być przydatna w badaniu źródeł infekcji oraz dróg szerzenia się Mycobacterium ulcerans.
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Detection of specific UL49 sequences of Marek's disease virus CVI988/Rispens strain using loop-mediated isothermal amplification
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Stromal-Epithelial Cross-Talk in the Intestinal Stem Cell Niche is Mediated by a Regulatory Network that Involves TGF-Beta and CTGF
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Synthesis of thermally responsive cylindrical molecular brushes via a combination of nitroxide-mediated radical polymerization and “grafting onto” strategy
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Microemulsion-Mediated Synthesis and Properties of Uniform Ln:CaWO4(Ln = Eu, Dy) Nanophosphors with Multicolor Luminescence for Optical and CT Imaging
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Towards plant-mediated chemistry – Au nanoparticles obtained using aqueous extract of Rosa damascena and their biological activity in vitro
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Potassium trimethylsilanolate-mediated conversion of dialkyl phosphonates to their anhydrous potassium monoalkyl phosphonates under mild, non-aqueous conditions
PublicationPrzedstawiona została metoda syntezy bezwodnych soli potasowych fosfonianów monoalkilu z odpowiednich fosfonianów dialkilu w reakcji z trimetylosilanolanem potasu w bezwodnym eterze i chlorku metylenu. Metoda ta może być również stosowana dla makrocyklicznych kaliks[4]arenów z wbudowanymi grupami fosfonowymi na górnej obręczy; preferowanym rozpuszczalnikiem jest w tym przypadku bezwodny THF. Zaletą opisanej metody są łagodne warunki...
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Use of rapid immunochromatographic tests and PCR for the detection of vaccine viruses (CPV, CDV CAV) in faeces and conjunctival sac swabs of vaccinated dogs
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Validation of Real-time PCR Reference Genes of Muscle Metabolism in Harvested Spiny-Cheek Crayfish (Faxonius limosus) Exposed to Seasonal Variation
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Discovery and characterization of RecA protein of thermophilic bacterium Thermus thermophilus MAT72 phage Tt72 that increases specificity of a PCR-based DNA amplification
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Superoxide dismutase is upregulated in Staphylococcus aureus following protoporphyrin-mediated photodynamic inactivation and does not directly influence the response to photodynamic treatment
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The Mechanism of the Osteoprotective Action of a Polyphenol-Rich Aronia melanocarpa Extract during Chronic Exposure to Cadmium is Mediated by the Oxidative Defense System
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Probability of second live birth after first natural and medically assisted reproduction‐mediated live birth: A historical cohort study
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Biochar-mediated transformation of titanium dioxide nanoparticles concerning TiO2NPs-biochar interactions, plant traits and tissue accumulation to cell translocation
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Csk homologous kinase (CHK), unlike Csk, enhances MAPK activation via Ras-mediated signaling in a Src-independent manner
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