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Search results for: RPOE SIGMA FACTOR
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublicationEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublicationTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...
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Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
PublicationThe outer membrane (OM) of Gram-negative bacteria is asymmetric due to the presence of lipopolysaccharide (LPS) facing the outer leaflet of the OM and phospholipids facing the periplasmic side. LPS is essential for bacterial viability, since it provides a permeability barrier and is a major virulence determinant in pathogenic bacteria. In Escherichia coli, several steps of LPS biosynthesis and assembly are regulated by the RpoE...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublicationDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublicationThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublicationEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
PublicationMass spectrometric analyses of lipopolysaccharide (LPS) from isogenic Escherichia coli strains with nonpolar mutations in the waa locus or overexpression of their cognate genes revealed that waaZ and waaS are the structural genes required for the incorporation of the third 3-deoxy-α-D-manno-oct-2-ulosonic acid (Kdo) linked to Kdo disaccharide and rhamnose, respectively. The incorporation of rhamnose requires prior sequential incorporation...
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Small regulatory bacterial RNAs regulating the envelope stress response
PublicationMost bacteria encode a large repertoire of RNA-based regulatory mechanisms. Recent discoveries have revealed that the expression of many genes is controlled by a plethora of base-pairing noncoding small regulatory RNAs (sRNAs), regulatory RNA-binding proteins and RNA-degrading enzymes. Some of these RNA-based regulated processes respond to stress conditions and are involved in the maintenance of cellular homeostasis. They achieve...
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The Implementation of the Taxonomic Spatial Measure of Development in the Analysis of Convergence in the Standard of Living
PublicationThe main goal of this paper is to analyse the existence of the social convergence in the European Union between 19952012. The social convergence refers to a reduction in the dispersion of the standard of living across countries. A taxonomic spatial measure of development was used as the standard of living approximation. The use of the new approach proposed by E. Antczak allowed for an explanation of the disparities in the analysed...
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Biofilm formation and its regulation in Staphylococcus aureus
PublicationInfections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and...
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Analog fault signature based on sigma-delta modulation and oscillation-test methodology.
PublicationW artykule dokonano przeglądu prac z zakresu testowania układów elektronicznych metodą oscylacyjną. Wskazując na niedostatki aktualnie stosowanej techniki testowania oscylacyjnego zaproponowano nową sygnaturę uszkodzeń dla układów analogowych. Proponowany parametr diagnostyczny jest wydobywany z odpowiedzi czasowej oscylatora testującego, w układzie złożonym z detektora szczytowego, modulatora sigma-delta oraz licznika rewersyjnego....
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Uwarunkowania aplikacyjne koncepcji Six Sigma w sektorze MŚP
PublicationArtykuł porusza istotne, z punktu widzenia podnoszenia konkurencyjności sektora MŚP a rzadko przedstawiane w literaturze krajowej, zagadnienie wykorzystania w tej grupie przedsiębiorstw koncepcji Six Sigma. Obok charakterystycznych cech MŚP wywierających wpływ na zastosowanie w nich metodyki Six Sigma, przedstawiono związki tej koncepcji z systemami zarządzania jakością.
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Assessment of preparedness for Lean Six Sigma projects in small and medium-sized organizations
PublicationThe article presents a proposal for the maturity indicator of Lean Six Sigma projects within the framework of the original comprehensive methodology of Lean Six Sigma implementation dedicated to the SME sector. The indicator allows an LSS project to be matched to the real needs and preparedness of any small or medium-sized organization. The indicator was designed as a result of pilot research conducted in Polish and French SMEs...
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Elements of six sigma methodoloygy in polish smes. the pomeranian region context
PublicationIn this article, it has been suggested that the effectiveness of the design and implementation of the process approach in the SMEs sector affects the effectiveness and efficiency of the application of elements of the Six Sigma ™ concept. The selected results of the research have been presented to answer the question to what extent small and medium-sized manufacturing, service and production - service companies in Pomeranian region...
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How to adapt Lean Six Sigma to improve performance of SMEs of manufacturing sector
PublicationThe main objective of the paper is to present the utilitarian effect of the adaptation of the Lean Six Sigma concept and its implementation in the small and medium-sized enterprises of the manufacturing sector. The original methodology was developed on the basis of research carried out in the Polish and French enterprises from 2009 to 2011. In the case of SMEs, the use of the concept of Lean Six Sigma is much less common, as...
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Six Sigma w małych i średnich przedsiębiorstwach. Puste hasło czy konieczność?
PublicationW artykule przedstawiono aspekty wykorzystania w sektorze małych i średnich przedsiębiorstw (MŚP) elementów koncepcji Six Sigma. Celem tego opracowania jest ocena potrzeb i uwarunkowań aplikacyjnych koncepcji Six Sigma w sektorze MŚP, zwłaszcza w nawiązaniu do bazujących na podejściu procesowym systemów zarządzania jakością. Zaprezentowano charakterystyczne cechy mniejszych organizacji, stanowiące w kontekście programów Six Sigma...
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Wsparcie informatyczne koncepcji Lean Management oraz Six Sigma
PublicationW rozdziale tym przedstawiono kluczowe aspekty integracji dwóch najważniejszych obecnie koncepcji optymalizacji procesów w organizacjach produkujących wyroby materialne oraz świadczących usługi – Six Sigma i Lean Manufacturing. Szczególną uwagę zwrócono na uwarunkowania wsparcia informatycznego tych koncepcji w mniejszych organizacjach.
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Simulation analysis of a production process with selected six sigma ratios
PublicationComputer technologies allow more and more to model as well as to perform simulation experiments of various processes. The simulation analysis provides a better understanding of the interdependencies between various stages of production processes.The results of simulation studies were presented, the aim of them was to show the opportunities of the analysis of the process according to the scenarios and variants developed in connection...